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Structural Characterization of the Mechanosensitive Channel Candidate MCA2 from Arabidopsis thaliana

Overview of attention for article published in PLOS ONE, January 2014
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  • Good Attention Score compared to outputs of the same age (73rd percentile)
  • Above-average Attention Score compared to outputs of the same age and source (64th percentile)

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6 X users

Citations

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30 Dimensions

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55 Mendeley
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Title
Structural Characterization of the Mechanosensitive Channel Candidate MCA2 from Arabidopsis thaliana
Published in
PLOS ONE, January 2014
DOI 10.1371/journal.pone.0087724
Pubmed ID
Authors

Hideki Shigematsu, Kazuko Iida, Masataka Nakano, Pratima Chaudhuri, Hidetoshi Iida, Kuniaki Nagayama

Abstract

Mechanosensing in plants is thought to be governed by sensory complexes containing a Ca²⁺-permeable, mechanosensitive channel. The plasma membrane protein MCA1 and its paralog MCA2 from Arabidopsis thaliana are involved in mechanical stress-induced Ca²⁺ influx and are thus considered as candidates for such channels or their regulators. Both MCA1 and MCA2 were functionally expressed in Sf9 cells using a baculovirus system in order to elucidate their molecular natures. Because of the abundance of protein in these cells, MCA2 was chosen for purification. Purified MCA2 in a detergent-solubilized state formed a tetramer, which was confirmed by chemical cross-linking. Single-particle analysis of cryo-electron microscope images was performed to depict the overall shape of the purified protein. The three-dimensional structure of MCA2 was reconstructed at a resolution of 26 Å from 5,500 particles and appears to comprise a small transmembrane region and large cytoplasmic region.

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X Demographics

The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 2%
France 1 2%
Unknown 53 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 15 27%
Student > Ph. D. Student 12 22%
Student > Master 7 13%
Student > Bachelor 6 11%
Professor 1 2%
Other 3 5%
Unknown 11 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 25 45%
Biochemistry, Genetics and Molecular Biology 11 20%
Engineering 2 4%
Chemistry 2 4%
Physics and Astronomy 1 2%
Other 2 4%
Unknown 12 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 September 2014.
All research outputs
#6,401,232
of 22,741,406 outputs
Outputs from PLOS ONE
#76,823
of 194,090 outputs
Outputs of similar age
#76,493
of 307,315 outputs
Outputs of similar age from PLOS ONE
#1,923
of 5,605 outputs
Altmetric has tracked 22,741,406 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 194,090 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.1. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 307,315 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 5,605 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.