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Cloning, Production and Characterization of a Glycoside Hydrolase Family 7 Enzyme from the Gut Microbiota of the Termite Coptotermes curvignathus

Overview of attention for article published in Molecular Biotechnology, June 2017
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Title
Cloning, Production and Characterization of a Glycoside Hydrolase Family 7 Enzyme from the Gut Microbiota of the Termite Coptotermes curvignathus
Published in
Molecular Biotechnology, June 2017
DOI 10.1007/s12033-017-0015-x
Pubmed ID
Authors

James Sy-Keen Woon, Patricia Jie Hung King, Mukram Mohamed Mackeen, Nor Muhammad Mahadi, Wan Mohd Khairulikhsan Wan Seman, William J. Broughton, Abdul Munir Abdul Murad, Farah Diba Abu Bakar

Abstract

Coptotermes curvignathus is a termite that, owing to its ability to digest living trees, serves as a gold mine for robust industrial enzymes. This unique characteristic reflects the presence of very efficient hydrolytic enzyme systems including cellulases. Transcriptomic analyses of the gut of C. curvignathus revealed that carbohydrate-active enzymes (CAZy) were encoded by 3254 transcripts and that included 69 transcripts encoding glycoside hydrolase family 7 (GHF7) enzymes. Since GHF7 enzymes are useful to the biomass conversion industry, a gene encoding for a GHF7 enzyme (Gh1254) was synthesized, sub-cloned and expressed in the methylotrophic yeast Pichia pastoris. Expressed GH1254 had an apparent molecular mass of 42 kDa, but purification was hampered by its low expression levels in shaken flasks. To obtain more of the enzyme, GH1254 was produced in a bioreactor that resulted in a fourfold increase in crude enzyme levels. The purified enzyme was active towards soluble synthetic substrates such as 4-methylumbelliferyl-β-D-cellobioside, 4-nitrophenyl-β-D-cellobioside and 4-nitrophenyl-β-D-lactoside but was non-hydrolytic towards Avicel or carboxymethyl cellulose. GH1254 catalyzed optimally at 35 °C and maintained 70% of its activity at 25 °C. This enzyme is thus potentially useful in food industries employing low-temperature conditions.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 33 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 30%
Student > Master 8 24%
Student > Ph. D. Student 5 15%
Professor > Associate Professor 2 6%
Student > Doctoral Student 1 3%
Other 5 15%
Unknown 2 6%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 12 36%
Agricultural and Biological Sciences 9 27%
Immunology and Microbiology 4 12%
Chemistry 2 6%
Nursing and Health Professions 1 3%
Other 3 9%
Unknown 2 6%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 June 2017.
All research outputs
#14,939,304
of 22,977,819 outputs
Outputs from Molecular Biotechnology
#622
of 972 outputs
Outputs of similar age
#188,435
of 316,526 outputs
Outputs of similar age from Molecular Biotechnology
#5
of 12 outputs
Altmetric has tracked 22,977,819 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 972 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 33rd percentile – i.e., 33% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 316,526 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.