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Mendeley readers
| Title |
Overproduction of valuable methoxylated flavones in induced tetraploid plants of Dracocephalum kotschyi Boiss
|
|---|---|
| Published in |
Botanical Studies, February 2014
|
| DOI | 10.1186/1999-3110-55-22 |
| Pubmed ID | |
| Authors |
Ali Akbar Zahedi, Bahman Hosseini, Mohammad Fattahi, Esmail Dehghan, Hadi Parastar, Hadi Madani |
| Abstract |
Ploidy manipulation is considered an efficient method to increase production potential of medicinally important compounds. Dracocephalum kotschyi Boiss. is an endangered medicinal plant of Iran. Various concentrations of colchicine (0.05, 0.10, 0.20, and 0.50% w/v) were applied to shoot apical meristems of D. kotschyi seedlings in two and four-leaf stages to induce tetraploidy. According to the results, 0.5% (w/v) of colchicine can be effective for polyploidy induction in D. kotschyi. Putative tetraploids were selected by morphological and microscopic characteristics and their ploidy level was confirmed by flow cytometry analysis and chromosome counting. The chromosome number of original diploid plant was confirmed to be 2n = 2× = 20 whereas that of the tetraploid plant was 2n = 4× = 40. Tetraploid and mixoploid plants showed different morphological, physiological and microscopic characteristics from those of diploid counterparts. The total content of flavonoids was increased from 1583.28 in diploids to 1890.07 (μg/g DW) in stable tetraploids. High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) confirmed over accumulation of methoxylated hydroxyflavones in solid tetraploid plants of D. kotschyi. |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 35 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 7 | 20% |
| Student > Ph. D. Student | 6 | 17% |
| Lecturer | 4 | 11% |
| Student > Master | 3 | 9% |
| Student > Doctoral Student | 2 | 6% |
| Other | 5 | 14% |
| Unknown | 8 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 46% |
| Unspecified | 2 | 6% |
| Biochemistry, Genetics and Molecular Biology | 2 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Physics and Astronomy | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 11 | 31% |