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Mendeley readers
Chapter title |
Blotting from PhastGel to Membranes by Ultrasound
|
---|---|
Chapter number | 25 |
Book title |
Western Blotting
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2694-7_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2693-0, 978-1-4939-2694-7
|
Authors |
Joseph Kost, Aharon Azagury |
Editors |
Biji T. Kurien, R. Hal Scofield |
Abstract |
Ultrasound based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm(2)) was sufficient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500-106,000 Da) and 14C-labeled Rainbow protein molecular weight markers (14,300-200,000 Da). |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Postgraduate | 1 | 20% |
Student > Master | 1 | 20% |
Unknown | 3 | 60% |
Readers by discipline | Count | As % |
---|---|---|
Chemical Engineering | 1 | 20% |
Engineering | 1 | 20% |
Unknown | 3 | 60% |