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Hematopoietic Stem Cell Protocols

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Cover of 'Hematopoietic Stem Cell Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 The Hematopoietic Stem Cell Landscape
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    Chapter 2 Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow
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    Chapter 3 Hematopoietic Stem Cell Protocols
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    Chapter 4 Hematopoietic Stem and Progenitor Cell Mobilization in Mice
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    Chapter 5 Cell cycle measurement of mouse hematopoietic stem/progenitor cells.
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    Chapter 6 Flow Cytometric Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells
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    Chapter 7 Gene Expression Profiling of Hematopoietic Stem Cells (HSCs)
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    Chapter 8 Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells.
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    Chapter 9 DNA methylation profiling of hematopoietic stem cells.
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    Chapter 10 Metabolic Characterization of Hematopoietic Stem Cells
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    Chapter 11 Nanoproteomic Assays on Hematopoietic Stem Cells
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    Chapter 12 Hematopoietic Differentiation of Pluripotent Stem Cells in Culture
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    Chapter 13 Ex Vivo Assays to Study Self-Renewal, Long-Term Expansion, and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells
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    Chapter 14 Ex vivo expansion of murine and human hematopoietic stem cells.
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    Chapter 15 Using Microfluidics to Investigate Hematopoietic Stem Cell and Microniche Interactions at the Single Cell Level
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    Chapter 16 Five-Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells
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    Chapter 17 Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull
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    Chapter 18 Immunodeficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development
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    Chapter 19 Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells
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    Chapter 20 Retroviral transduction of murine and human hematopoietic progenitors and stem cells.
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    Chapter 21 Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells
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    Chapter 22 High-throughput genomic mapping of vector integration sites in gene therapy studies.
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    Chapter 23 Barcoded Vector Libraries and Retroviral or Lentiviral Barcoding of Hematopoietic Stem Cells
Attention for Chapter 14: Ex vivo expansion of murine and human hematopoietic stem cells.
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Chapter title
Ex vivo expansion of murine and human hematopoietic stem cells.
Chapter number 14
Book title
Hematopoietic Stem Cell Protocols
Published in
Methods in molecular biology, June 2014
DOI 10.1007/978-1-4939-1133-2_14
Pubmed ID
Book ISBNs
978-1-4939-1132-5, 978-1-4939-1133-2
Authors

Doan PL, Chute JP, Doan, Phuong L., Chute, John P., Phuong L. Doan, John P. Chute

Editors

Kevin D. Bunting, Cheng-Kui Qu

Abstract

Hematopoietic stem cells have the capacity to self-renew and give rise to the entirety of the mature blood and immune system throughout the lifespan of an organism. Here, we describe methods to isolate and culture murine bone marrow (BM) CD34(-)ckit(+)Sca1(+)Lineage(-) (CD34(-)KSL) hematopoietic stem cells (HSCs). We also describe a method to measure functional HSC content via the competitive repopulation assay. Furthermore, we summarize methods to isolate and culture human CD34(+)CD38(-)Lineage(-) cells which are enriched for human hematopoietic stem and progenitor cells.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 44%
Student > Ph. D. Student 1 11%
Other 1 11%
Student > Bachelor 1 11%
Student > Doctoral Student 1 11%
Other 0 0%
Unknown 1 11%
Readers by discipline Count As %
Medicine and Dentistry 3 33%
Biochemistry, Genetics and Molecular Biology 2 22%
Nursing and Health Professions 1 11%
Agricultural and Biological Sciences 1 11%
Neuroscience 1 11%
Other 0 0%
Unknown 1 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 December 2014.
All research outputs
#20,245,139
of 22,772,779 outputs
Outputs from Methods in molecular biology
#9,863
of 13,090 outputs
Outputs of similar age
#192,171
of 227,606 outputs
Outputs of similar age from Methods in molecular biology
#51
of 107 outputs
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So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 107 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.