Title |
Toxoplasma depends on lysosomal consumption of autophagosomes for persistent infection
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Published in |
Nature Microbiology, June 2017
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DOI | 10.1038/nmicrobiol.2017.96 |
Pubmed ID | |
Authors |
Manlio Di Cristina, Zhicheng Dou, Matteo Lunghi, Geetha Kannan, My-Hang Huynh, Olivia L. McGovern, Tracey L. Schultz, Aric J. Schultz, Alyssa J. Miller, Beth M. Hayes, Wouter van der Linden, Carla Emiliani, Matthew Bogyo, Sébastien Besteiro, Isabelle Coppens, Vern B. Carruthers |
Abstract |
Globally, nearly 2 billion people are infected with the intracellular protozoan Toxoplasma gondii(1). This persistent infection can cause severe disease in immunocompromised people and is epidemiologically linked to major mental illnesses(2) and cognitive impairment(3). There are currently no options for curing this infection. The lack of effective therapeutics is due partly to a poor understanding of the essential pathways that maintain long-term infection. Although it is known that Toxoplasma replicates slowly within intracellular cysts demarcated with a cyst wall, precisely how it sustains itself and remodels organelles in this niche is unknown. Here, we identify a key role for proteolysis within the parasite lysosomal organelle (the vacuolar compartment or VAC) in turnover of autophagosomes and persistence during neural infection. We found that disrupting a VAC-localized cysteine protease compromised VAC digestive function and markedly reduced chronic infection. Death of parasites lacking the VAC protease was preceded by accumulation of undigested autophagosomes in the parasite cytoplasm. These findings suggest an unanticipated function for parasite lysosomal degradation in chronic infection, and identify an intrinsic role for autophagy in the T. gondii parasite and its close relatives. This work also identifies a key element of Toxoplasma persistence and suggests that VAC proteolysis is a prospective target for pharmacological development. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 7 | 33% |
United States | 3 | 14% |
Germany | 2 | 10% |
Russia | 1 | 5% |
France | 1 | 5% |
India | 1 | 5% |
Unknown | 6 | 29% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 11 | 52% |
Scientists | 9 | 43% |
Science communicators (journalists, bloggers, editors) | 1 | 5% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 69 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 20 | 29% |
Researcher | 10 | 14% |
Student > Bachelor | 8 | 12% |
Student > Master | 6 | 9% |
Professor > Associate Professor | 4 | 6% |
Other | 13 | 19% |
Unknown | 8 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 21 | 30% |
Biochemistry, Genetics and Molecular Biology | 19 | 28% |
Immunology and Microbiology | 11 | 16% |
Chemistry | 3 | 4% |
Medicine and Dentistry | 3 | 4% |
Other | 4 | 6% |
Unknown | 8 | 12% |