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Genome Editing in Animals

Overview of attention for book
Cover of 'Genome Editing in Animals'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Construction and Evaluation of Zinc Finger Nucleases
  3. Altmetric Badge
    Chapter 2 Current Overview of TALEN Construction Systems
  4. Altmetric Badge
    Chapter 3 CRISPR/Cas9
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    Chapter 4 Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse
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    Chapter 5 Genome Editing of Mouse by Cytoplasmic Injection
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    Chapter 6 Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing SgRNA/Cas9 Expressing Plasmids
  8. Altmetric Badge
    Chapter 7 Genome Editing in Mouse and Rat by Electroporation
  9. Altmetric Badge
    Chapter 8 Generation of Knock-in Mouse by Genome Editing
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    Chapter 9 Genome Editing of Rat
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    Chapter 10 Gene Targeting in Rabbits: Single-Step Generation of Knock-out Rabbits by Microinjection of CRISPR/Cas9 Plasmids
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    Chapter 11 Genome Editing of Pig
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    Chapter 12 Genome Editing of Monkey
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    Chapter 13 Genome Editing Mediated by Primordial Germ Cell in Chicken
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    Chapter 14 CRISPR/Cas9-Mediated Targeted Knockin of Exogenous Reporter Genes in Zebrafish
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    Chapter 15 Genome Editing of Medaka
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    Chapter 16 A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders Using the CRISPR-Cas System
  18. Altmetric Badge
    Chapter 17 Genome Editing of Silkworms
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    Chapter 18 Genome Editing in the Cricket, Gryllus bimaculatus
  20. Altmetric Badge
    Chapter 19 Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease
  21. Altmetric Badge
    Chapter 20 Genome Editing of C. elegans
Attention for Chapter 1: Construction and Evaluation of Zinc Finger Nucleases
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Chapter title
Construction and Evaluation of Zinc Finger Nucleases
Chapter number 1
Book title
Genome Editing in Animals
Published in
Methods in molecular biology, June 2017
DOI 10.1007/978-1-4939-7128-2_1
Pubmed ID
Book ISBNs
978-1-4939-7127-5, 978-1-4939-7128-2
Authors

Hiroshi Ochiai, Takashi Yamamoto, Ochiai, Hiroshi, Yamamoto, Takashi

Editors

Izuho Hatada

Abstract

Zinc-finger nucleases (ZFNs) are programmable nucleases that have opened the door to the genome editing era. The construction of ZFNs recognizing a target sequence of interest is laborious, and has not been widely used recently. However, key ZFN patents are expiring over the next 2-4 years, enabling a wide range of deployments for clinical and industrial applications. This article introduces a ZFN construction protocol that uses bacterial one-hybrid (B1H) selection and single-stranded annealing (SSA) assay.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 8 33%
Student > Master 4 17%
Professor 2 8%
Researcher 2 8%
Student > Ph. D. Student 1 4%
Other 0 0%
Unknown 7 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 42%
Agricultural and Biological Sciences 3 13%
Social Sciences 1 4%
Medicine and Dentistry 1 4%
Neuroscience 1 4%
Other 1 4%
Unknown 7 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 September 2021.
All research outputs
#15,466,074
of 22,982,639 outputs
Outputs from Methods in molecular biology
#5,379
of 13,149 outputs
Outputs of similar age
#198,776
of 316,289 outputs
Outputs of similar age from Methods in molecular biology
#97
of 280 outputs
Altmetric has tracked 22,982,639 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,149 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 316,289 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 280 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.