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Universal sample preparation method for proteome analysis

Overview of attention for article published in Nature Methods, April 2009
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (97th percentile)
  • High Attention Score compared to outputs of the same age and source (93rd percentile)

Mentioned by

news
2 news outlets
blogs
3 blogs
twitter
1 X user
patent
45 patents
wikipedia
1 Wikipedia page
q&a
1 Q&A thread

Citations

dimensions_citation
6466 Dimensions

Readers on

mendeley
3416 Mendeley
citeulike
10 CiteULike
connotea
3 Connotea
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Title
Universal sample preparation method for proteome analysis
Published in
Nature Methods, April 2009
DOI 10.1038/nmeth.1322
Pubmed ID
Authors

Jacek R Wiśniewski, Alexandre Zougman, Nagarjuna Nagaraj, Matthias Mann

Abstract

We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3,416 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 35 1%
Germany 16 <1%
United Kingdom 14 <1%
Spain 8 <1%
Netherlands 6 <1%
South Africa 5 <1%
India 5 <1%
Brazil 4 <1%
Italy 3 <1%
Other 29 <1%
Unknown 3291 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 867 25%
Researcher 690 20%
Student > Master 395 12%
Student > Bachelor 276 8%
Student > Doctoral Student 182 5%
Other 435 13%
Unknown 571 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 1030 30%
Biochemistry, Genetics and Molecular Biology 817 24%
Chemistry 279 8%
Medicine and Dentistry 147 4%
Immunology and Microbiology 98 3%
Other 346 10%
Unknown 699 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 54. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 March 2024.
All research outputs
#803,289
of 26,017,215 outputs
Outputs from Nature Methods
#1,048
of 5,401 outputs
Outputs of similar age
#1,853
of 111,000 outputs
Outputs of similar age from Nature Methods
#3
of 46 outputs
Altmetric has tracked 26,017,215 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,401 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 36.7. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 111,000 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.