| Chapter title |
Vaccination Using Gene-Gun Technology.
|
|---|---|
| Chapter number | 22 |
| Book title |
Malaria Vaccines
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2815-6_22 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2814-9, 978-1-4939-2815-6
|
| Authors |
Bergmann-Leitner, Elke S, Leitner, Wolfgang W, Elke S. Bergmann-Leitner, Wolfgang W. Leitner |
| Editors |
Ashley Vaughan |
| Abstract |
DNA vaccines against infection with Plasmodium have been highly successful in rodent models of malaria and have shown promise in the very limited number of clinical trials conducted so far. The vaccine platform is highly attractive for numerous reasons, such as low cost and a very favorable safety profile. Gene gun delivery of DNA plasmids drastically reduces the vaccine dose and does not only have the potential to make vaccines more accessible and affordable, but also simplifies (a) the testing of novel antigens as vaccine candidates, (b) the testing of antigen combinations, and (c) the co-delivery of antigens with molecular adjuvants such as cytokines or costimulatory molecules. Described in this chapter are the preparation of the inoculum (i.e., DNA plasmids attached to gold particles, coating to the inside of plastic tubing also referred to as gene gun "bullets" or cartridges), the gene gun vaccination procedure, and the challenge of mice with Plasmodium berghei parasites to test the efficacy of the experimental vaccine. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 29 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 5 | 17% |
| Student > Bachelor | 5 | 17% |
| Professor > Associate Professor | 3 | 10% |
| Student > Doctoral Student | 2 | 7% |
| Student > Postgraduate | 2 | 7% |
| Other | 6 | 21% |
| Unknown | 6 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 28% |
| Agricultural and Biological Sciences | 3 | 10% |
| Economics, Econometrics and Finance | 2 | 7% |
| Nursing and Health Professions | 2 | 7% |
| Unspecified | 1 | 3% |
| Other | 6 | 21% |
| Unknown | 7 | 24% |