Title |
Isolation and molecular characterization of Thraustochytrium strain isolated from Antarctic Peninsula and its biotechnological potential in the production of fatty acids
|
---|---|
Published in |
Brazilian Journal of Microbiology, June 2017
|
DOI | 10.1016/j.bjm.2017.01.011 |
Pubmed ID | |
Authors |
Esteban Caamaño, Lyliam Loperena, Ivonne Hinzpeter, Paulina Pradel, Felipe Gordillo, Gino Corsini, Mario Tello, Paris Lavín, Alex R. González |
Abstract |
Thraustochytrids are unicellular protists belonging to the Labyrinthulomycetes class, which are characterized by the presence of a high lipid content that could replace conventional fatty acids. They show a wide geographic distribution, however their diversity in the Antarctic Region is rather scarce. The analysis based on the complete sequence of 18S rRNA gene showed that strain 34-2 belongs to the species Thraustochytrium kinnei, with 99% identity. The total lipid profile shows a wide range of saturated fatty acids with abundance of palmitic acid (16:0), showing a range of 16.1-19.7%. On the other hand, long-chain polyunsaturated fatty acids, mainly docosahexaenoic acid and eicosapentaenoic acid are present in a range of 24-48% and 6.1-9.3%, respectively. All factors analyzed in cells (biomass, carbon consumption and lipid content) changed with variations of culture temperature (10°C and 25°C). The growth in glucose at a temperature of 10°C presented the most favorable conditions to produce omega-3fatty acid. This research provides the identification and characterization of a Thraustochytrids strain, with a total lipid content that presents potential applications in the production of nutritional supplements and as well biofuels. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Science communicators (journalists, bloggers, editors) | 2 | 50% |
Scientists | 1 | 25% |
Members of the public | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 161 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 21 | 13% |
Researcher | 10 | 6% |
Student > Ph. D. Student | 7 | 4% |
Student > Master | 6 | 4% |
Other | 4 | 2% |
Other | 12 | 7% |
Unknown | 101 | 63% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 19 | 12% |
Biochemistry, Genetics and Molecular Biology | 13 | 8% |
Chemical Engineering | 6 | 4% |
Engineering | 4 | 2% |
Environmental Science | 4 | 2% |
Other | 13 | 8% |
Unknown | 102 | 63% |