Chapter title |
From Structure to Function: A Comprehensive Compendium of Tools to Unveil Protein Domains and Understand Their Role in Cytokinesis.
|
---|---|
Chapter number | 24 |
Book title |
Yeast Cytokinesis
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3145-3_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3144-6, 978-1-4939-3145-3
|
Authors |
Rincon, Sergio A, Paoletti, Anne, Sergio A Rincon, Anne Paoletti |
Editors |
Alberto Sanchez-Diaz, Pilar Perez |
Abstract |
Unveiling the function of a novel protein is a challenging task that requires careful experimental design. Yeast cytokinesis is a conserved process that involves modular structural and regulatory proteins. For such proteins, an important step is to identify their domains and structural organization. Here we briefly discuss a collection of methods commonly used for sequence alignment and prediction of protein structure that represent powerful tools for the identification homologous domains and design of structure-function approaches to test experimentally the function of multi-domain proteins such as those implicated in yeast cytokinesis. |
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