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Derivation of therapeutic lung spheroid cells from minimally invasive transbronchial pulmonary biopsies

Overview of attention for article published in Respiratory Research, June 2017
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#4 of 1,429)
  • High Attention Score compared to outputs of the same age (97th percentile)
  • High Attention Score compared to outputs of the same age and source (98th percentile)

Mentioned by

news
15 news outlets
blogs
2 blogs
twitter
5 tweeters
googleplus
2 Google+ users

Citations

dimensions_citation
11 Dimensions

Readers on

mendeley
25 Mendeley
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Title
Derivation of therapeutic lung spheroid cells from minimally invasive transbronchial pulmonary biopsies
Published in
Respiratory Research, June 2017
DOI 10.1186/s12931-017-0611-0
Pubmed ID
Authors

Phuong-Uyen C. Dinh, Jhon Cores, M. Taylor Hensley, Adam C. Vandergriff, Junnan Tang, Tyler A. Allen, Thomas G. Caranasos, Kenneth B. Adler, Leonard J. Lobo, Ke Cheng

Abstract

Resident stem and progenitor cells have been identified in the lung over the last decade, but isolation and culture of these cells remains a challenge. Thus, although these lung stem and progenitor cells provide an ideal source for stem-cell based therapy, mesenchymal stem cells (MSCs) remain the most popular cell therapy product for the treatment of lung diseases. Surgical lung biopsies can be the tissue source but such procedures carry a high risk of mortality. In this study we demonstrate that therapeutic lung cells, termed "lung spheroid cells" (LSCs) can be generated from minimally invasive transbronchial lung biopsies using a three-dimensional culture technique. The cells were then characterized by flow cytometry and immunohistochemistry. Angiogenic potential was tested by in-vitro HUVEC tube formation assay. In-vivo bio- distribution of LSCs was examined in athymic nude mice after intravenous delivery. From one lung biopsy, we are able to derive >50 million LSC cells at Passage 2. These cells were characterized by flow cytometry and immunohistochemistry and were shown to represent a mixture of lung stem cells and supporting cells. When introduced systemically into nude mice, LSCs were retained primarily in the lungs for up to 21 days. Here, for the first time, we demonstrated that direct culture and expansion of human lung progenitor cells from pulmonary tissues, acquired through a minimally invasive biopsy, is possible and straightforward with a three-dimensional culture technique. These cells could be utilized in long-term expansion of lung progenitor cells and as part of the development of cell-based therapies for the treatment of lung diseases such as chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis (IPF).

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 28%
Researcher 4 16%
Student > Ph. D. Student 4 16%
Student > Doctoral Student 2 8%
Professor 2 8%
Other 3 12%
Unknown 3 12%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 24%
Medicine and Dentistry 5 20%
Immunology and Microbiology 3 12%
Agricultural and Biological Sciences 2 8%
Nursing and Health Professions 1 4%
Other 3 12%
Unknown 5 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 119. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 January 2018.
All research outputs
#107,949
of 12,376,381 outputs
Outputs from Respiratory Research
#4
of 1,429 outputs
Outputs of similar age
#5,702
of 269,990 outputs
Outputs of similar age from Respiratory Research
#1
of 58 outputs
Altmetric has tracked 12,376,381 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,429 research outputs from this source. They receive a mean Attention Score of 5.0. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 269,990 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 58 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.