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Detection of Diazotrophy in the Acetylene-Fermenting Anaerobe Pelobacter sp. Strain SFB93

Overview of attention for article published in Applied and Environmental Microbiology, June 2017
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (84th percentile)
  • Good Attention Score compared to outputs of the same age and source (78th percentile)

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19 tweeters

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Title
Detection of Diazotrophy in the Acetylene-Fermenting Anaerobe Pelobacter sp. Strain SFB93
Published in
Applied and Environmental Microbiology, June 2017
DOI 10.1128/aem.01198-17
Pubmed ID
Authors

Denise M. Akob, Shaun M. Baesman, John M. Sutton, Janna L. Fierst, Adam C. Mumford, Yesha Shrestha, Amisha T. Poret-Peterson, Stacy Bennett, Darren S. Dunlap, Karl B. Haase, Ronald S. Oremland

Abstract

Acetylene (C2H2) is a trace constituent of the present Earth's oxidizing atmosphere, reflecting a mix of terrestrial and marine emissions from anthropogenic, biomass burning, and unidentified biogenic sources. Fermentation of acetylene was serendipitously discovered during C2H2-block assays of N2O reductase, and Pelobacter acetylenicus was shown to grow on C2H2 via acetylene hydratase (AH). AH is a W-containing, catabolic, low redox potential enzyme that unlike nitrogenase (N2ase) is specific for acetylene. Acetylene fermentation is a rare metabolism that is well-characterized only in P. acetylenicus DSM3246 and DSM3247, and Pelobacter sp. strain SFB93. To better understand the genetic controls on AH activity, we sequenced the genomes of the three acetylene-fermenting Pelobacter strains. Genome assembly and annotation produced three novel genomes containing gene sequences for AH, with two copies being present in SFB93. In addition, gene sequences for all five compulsory genes for Mo-Fe nitrogenase were also present in the three genomes, indicating the co-occurrence of 2 acetylene-transformation pathways. Nitrogen fixation growth assays showed that DSM3426 could ferment acetylene in the absence of ammonium, but no ethylene was produced. However, SFB93 degraded acetylene, and in the absence of ammonium, produced ethylene indicating an active N2ase. Diazotrophic growth was observed under N2 but not in experimental controls incubated under Ar. SFB93 exhibits acetylene fermentation and nitrogen fixation, the only known biochemical mechanisms for acetylene transformation. Our results indicate complex interactions between N2ase and AH and suggest novel evolutionary pathways of these relic enzymes from early Earth to modern day.Importance Here we show that a single Pelobacter strain can grow via acetylene fermentation and carry out nitrogen fixation, using the only 2 enzymes known to transform acetylene. These findings provide new insights into acetylene transformations and adaptations for nutrient (C, N) and energy acquisition by microorganisms. Enhanced understanding of acetylene transformations in modern environments (i.e., extent, occurrence, rates, etc.) is important for using acetylene as a potential biomarker for extraterrestrial life and degradation of anthropogenic contaminants.

Twitter Demographics

The data shown below were collected from the profiles of 19 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Other 3 50%
Researcher 2 33%
Student > Master 1 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 67%
Environmental Science 1 17%
Earth and Planetary Sciences 1 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 March 2018.
All research outputs
#1,244,378
of 12,582,044 outputs
Outputs from Applied and Environmental Microbiology
#859
of 9,555 outputs
Outputs of similar age
#41,832
of 262,262 outputs
Outputs of similar age from Applied and Environmental Microbiology
#24
of 110 outputs
Altmetric has tracked 12,582,044 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,555 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.0. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 262,262 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 110 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.