Title |
Comparison of gene expression of Paramecium bursaria with and without Chlorella variabilissymbionts
|
---|---|
Published in |
BMC Genomics, March 2014
|
DOI | 10.1186/1471-2164-15-183 |
Pubmed ID | |
Authors |
Yuuki Kodama, Haruo Suzuki, Hideo Dohra, Manabu Sugii, Tatsuya Kitazume, Katsushi Yamaguchi, Shuji Shigenobu, Masahiro Fujishima |
Abstract |
The ciliate Paramecium bursaria harbors several hundred cells of the green-alga Chlorella sp. in their cytoplasm. Irrespective of the mutual relation between P. bursaria and the symbiotic algae, both cells retain the ability to grow without the partner. They can easily reestablish endosymbiosis when put in contact with each other. Consequently, P. bursaria is an excellent model for studying cell-cell interaction and the evolution of eukaryotic cells through secondary endosymbiosis between different protists. Despite the importance of this organism, no genomic resources have been identified for P. bursaria to date. This investigation compared gene expressions through RNA-Seq analysis and de novo transcriptome assembly of symbiont-free and symbiont-bearing host cells. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 3 | 3% |
United Kingdom | 2 | 2% |
Canada | 2 | 2% |
Ireland | 1 | <1% |
Unknown | 95 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 17 | 17% |
Researcher | 17 | 17% |
Student > Master | 14 | 14% |
Professor > Associate Professor | 10 | 10% |
Student > Bachelor | 8 | 8% |
Other | 15 | 15% |
Unknown | 22 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 53 | 51% |
Biochemistry, Genetics and Molecular Biology | 13 | 13% |
Environmental Science | 3 | 3% |
Immunology and Microbiology | 3 | 3% |
Computer Science | 2 | 2% |
Other | 3 | 3% |
Unknown | 26 | 25% |