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Combination of commercially available molecular assays and culture based methods in diagnosis of tuberculosis and drug resistant tuberculosis

Overview of attention for article published in Brazilian Journal of Microbiology, October 2017
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  • Above-average Attention Score compared to outputs of the same age and source (61st percentile)

Mentioned by

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5 tweeters

Citations

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2 Dimensions

Readers on

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35 Mendeley
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Title
Combination of commercially available molecular assays and culture based methods in diagnosis of tuberculosis and drug resistant tuberculosis
Published in
Brazilian Journal of Microbiology, October 2017
DOI 10.1016/j.bjm.2017.04.001
Pubmed ID
Authors

Lamprini Gkaravela, Matthaios Papadimitriou-Olivgeris, Antigoni Foka, Fevronia Kolonitsiou, Anastasia Spiliopoulou, Nikolaos Charokopos, Apostolos Voulgaridis, Maria Tsiamita, Markos Marangos, Evangelos D. Anastassiou, Iris Spiliopoulou

Abstract

Early diagnosis of tuberculosis is of major clinical importance. Among 4733 clinical specimens collected from 3363 patients and subjected to Ziehl-Neelsen microscopy, 4109 were inoculated onto Löwenstein-Jensen slants and 3139 in Bactec/9000MB. Polymerase Chain Reaction (PCR) was performed in 3139 specimens, whereas, a genotypic assay was directly applied in 93 Mycobacterium tuberculosis complex PCR-positive for isoniazid and rifampicin resistance detection specimens (GenoType MTBDRplus). Recovered M. tuberculosis isolates (64) as well as, 21 more sent from Regional Hospitals were tested for antimycobacterial resistance with a phenotypic (manual MGIT-SIRE) and a genotypic assay (GenoType MTBDRplus). PCR in the clinical specimens showed excellent specificity (97.4%) and accuracy (96.8%), good sensitivity (70.4%), but low positive predictive value (40.3%). MGIT-SIRE performed to M. tuberculosis did not confer a reliable result in 16 isolates. Of the remaining 69 isolates, 15 were resistant to streptomycin, seven to isoniazid, seven to ethambutol and five to rifampicin. GenoType MTBDRplus correctly detected isoniazid (seven) and rifampicin-resistant M. tuberculosis strains (five), showing an excellent performance overall (100%). Susceptibility results by the molecular assay applied directly to clinical specimens were identical to those obtained from recovered isolates of the corresponding patients. Combining molecular and conventional methods greatly contribute to early diagnosis and accurate susceptibility testing of tuberculosis.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 35 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 20%
Other 5 14%
Student > Master 5 14%
Researcher 4 11%
Professor 3 9%
Other 6 17%
Unknown 5 14%
Readers by discipline Count As %
Medicine and Dentistry 14 40%
Chemistry 3 9%
Biochemistry, Genetics and Molecular Biology 2 6%
Veterinary Science and Veterinary Medicine 1 3%
Nursing and Health Professions 1 3%
Other 6 17%
Unknown 8 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 May 2018.
All research outputs
#9,342,459
of 15,916,110 outputs
Outputs from Brazilian Journal of Microbiology
#197
of 481 outputs
Outputs of similar age
#135,814
of 268,346 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#5
of 13 outputs
Altmetric has tracked 15,916,110 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 481 research outputs from this source. They receive a mean Attention Score of 3.0. This one has gotten more attention than average, scoring higher than 57% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 268,346 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 61% of its contemporaries.