Chapter title |
ADP-Ribosylated Peptide Enrichment and Site Identification: The Phosphodiesterase-Based Method
|
---|---|
Chapter number | 7 |
Book title |
Poly(ADP-Ribose) Polymerase
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-6993-7_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6992-0, 978-1-4939-6993-7
|
Authors |
Casey M. Daniels, Shao-En Ong, Anthony K. L. Leung |
Abstract |
Protein ADP-ribosylation is a posttranslational modification (PTM) that plays an important role in all major cellular processes, including DNA repair, cellular signaling, and RNA metabolism. Site identification for this PTM has recently become possible through the development of several mass spectrometry-based methods, a critical step in understanding the regulatory role played by mono(ADP-ribose) (MAR), poly(ADP-ribose) (PAR), and the enzymes which make these modifications: poly(ADP-ribose) polymerases (PARPs), best known for their role in DNA repair and as targets for chemotherapeutic PARP inhibitors. Here, we have described our method for enriching and identifying ADP-ribosylation events through the use of a phosphodiesterase to digest protein-conjugated ADP-ribose down to its attachment structure, phosphoribose. We also include here a guide to choosing between collision-induced dissociation (CID)-, higher-energy collisional dissociation (HCD)-, and electron-transfer dissociation (ETD)-based peptide fragmentation for the identification of phosphoribosylated peptides. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 19 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 26% |
Researcher | 3 | 16% |
Student > Postgraduate | 2 | 11% |
Student > Master | 2 | 11% |
Lecturer | 1 | 5% |
Other | 4 | 21% |
Unknown | 2 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 26% |
Agricultural and Biological Sciences | 5 | 26% |
Chemistry | 2 | 11% |
Nursing and Health Professions | 1 | 5% |
Immunology and Microbiology | 1 | 5% |
Other | 1 | 5% |
Unknown | 4 | 21% |