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Mendeley readers
| Chapter title |
Quick Detection of DNase II-Type Breaks in Formalin-Fixed Tissue Sections
|
|---|---|
| Chapter number | 9 |
| Book title |
Fast Detection of DNA Damage
|
| Published in |
Methods in molecular biology, July 2017
|
| DOI | 10.1007/978-1-4939-7187-9_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7185-5, 978-1-4939-7187-9
|
| Authors |
Candace L. Minchew, Vladimir V. Didenko |
| Abstract |
Blunt-ended DNase II-type breaks with 5' hydroxyls are generated in phagocytic cells of any lineage during digestion of the engulfed DNA. These breaks indicate the ongoing active phagocytic reaction. They are produced by the acid deoxyribonuclease-DNase II which is the primary endonuclease responsible for DNA degradation after its engulfment.Here, we present an express approach that detects blunt-ended 5' OH DNA breaks in fixed tissue sections. The technique is simple to perform and takes only 60 min to complete. It can be useful in studies of the clearance of dying cells in oncological, inflammatory, and autoimmune disorders. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 20% |
| Professor > Associate Professor | 1 | 10% |
| Unspecified | 1 | 10% |
| Student > Postgraduate | 1 | 10% |
| Researcher | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 4 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 20% |
| Unspecified | 1 | 10% |
| Agricultural and Biological Sciences | 1 | 10% |
| Unknown | 6 | 60% |