Title |
Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus
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Published in |
Virology Journal, July 2017
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DOI | 10.1186/s12985-017-0792-7 |
Pubmed ID | |
Authors |
Yang Yang, Xiaodong Qin, Xiangle Zhang, Zhixun Zhao, Wei Zhang, Xueliang Zhu, Guozheng Cong, Yanmin Li, Zhidong Zhang |
Abstract |
Goatpox virus (GTPV) and sheeppox virus (SPPV), which belong to the Capripoxvirus (CaPV), are economically important pathogens of small ruminants. Therefore, a sensitive, specific and rapid diagnostic assay for detection of GTPV and SPPV is necessary to accurately and promptly control these diseases. Recombinase polymerase amplification (RPA) assays combined with a real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the CaPV G-protein-coupled chemokine receptor (GPCR) gene, respectively. The sensitivity of both CaPV real-time RPA assay and CaPV RPA LFD assay were 3 × 10(2) copies per reaction within 20 min at 38 °C. Both assays were highly specific for CaPV, with no cross-reactions with peste des petits ruminants virus, foot-and-mouth disease virus and Orf virus. The evaluation of the performance of these two assays with clinical sample (n = 107) showed that the CaPV real-time RPA assay and CaPV RPA LFD assay were able to specially detect SPPV or GTPV present in samples of ovine in liver, lung, kidney, spleen, skin and blood. This study provided a highly time-efficient and simple alternative for rapid detection of GTPV and SPPV. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Canada | 1 | 20% |
Spain | 1 | 20% |
Unknown | 3 | 60% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 2 | 40% |
Members of the public | 2 | 40% |
Science communicators (journalists, bloggers, editors) | 1 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 43 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 14% |
Researcher | 6 | 14% |
Student > Bachelor | 4 | 9% |
Student > Master | 4 | 9% |
Student > Doctoral Student | 2 | 5% |
Other | 7 | 16% |
Unknown | 14 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 14% |
Chemistry | 5 | 12% |
Veterinary Science and Veterinary Medicine | 4 | 9% |
Immunology and Microbiology | 4 | 9% |
Biochemistry, Genetics and Molecular Biology | 3 | 7% |
Other | 8 | 19% |
Unknown | 13 | 30% |