Title |
Defective Decatenation Checkpoint Function Is a Common Feature of Melanoma
|
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Published in |
Journal of Investigative Dermatology, June 2013
|
DOI | 10.1038/jid.2013.264 |
Pubmed ID | |
Authors |
Kelly Brooks, Kee Ming Chia, Loredana Spoerri, Pamela Mukhopadhyay, Matthew Wigan, Mitchell Stark, Sandra Pavey, Brian Gabrielli |
Abstract |
A hallmark of cancer is genomic instability that is considered to provide the adaptive capacity of cancers to thrive under conditions in which the normal precursors would not survive. Recent genomic analysis has revealed a very high degree of genomic instability in melanomas, although the mechanism by which this instability arises is not known. Here we report that a high proportion (68%) of melanoma cell lines are either partially (40%) or severely (28%) compromised for the G2 phase decatenation checkpoint that normally functions to ensure that the sister chromatids are able to separate correctly during mitosis. The consequence of this loss of checkpoint function is a severely reduced ability to partition the replicated genome in mitosis and thereby increase genomic instability. We also demonstrate that decatenation is dependent on both TopoIIα and β isoforms. The high incidence of decatenation checkpoint defect is likely to be a major contributor to the high level of genomic instability found in melanomas. |
X Demographics
Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 7 | 44% |
Student > Ph. D. Student | 2 | 13% |
Unspecified | 1 | 6% |
Student > Bachelor | 1 | 6% |
Other | 1 | 6% |
Other | 2 | 13% |
Unknown | 2 | 13% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 8 | 50% |
Agricultural and Biological Sciences | 2 | 13% |
Unspecified | 1 | 6% |
Psychology | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Other | 1 | 6% |
Unknown | 2 | 13% |