Title |
Single-molecule FRET of protein structure and dynamics - a primer
|
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Published in |
Journal of Nanobiotechnology, December 2013
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DOI | 10.1186/1477-3155-11-s1-s2 |
Pubmed ID | |
Authors |
Benjamin Schuler |
Abstract |
Single-molecule spectroscopy has developed into a widely used method for probing the structure, dynamics, and mechanisms of biomolecular systems, especially in combination with Förster resonance energy transfer (FRET). In this introductory tutorial, essential concepts and methods will be outlined, from the FRET process and the basic considerations for sample preparation and instrumentation to some key elements of data analysis and photon statistics. Different approaches for obtaining dynamic information over a wide range of timescales will be explained and illustrated with examples, including the quantitative analysis of FRET efficiency histograms, correlation spectroscopy, fluorescence trajectories, and microfluidic mixing. |
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Germany | 1 | 25% |
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Denmark | 1 | 25% |
Hungary | 1 | 25% |
Demographic breakdown
Type | Count | As % |
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Scientists | 3 | 75% |
Members of the public | 1 | 25% |
Mendeley readers
Geographical breakdown
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Bangladesh | 1 | <1% |
Germany | 1 | <1% |
Turkey | 1 | <1% |
Netherlands | 1 | <1% |
Korea, Republic of | 1 | <1% |
Sweden | 1 | <1% |
South Africa | 1 | <1% |
India | 1 | <1% |
Spain | 1 | <1% |
Other | 1 | <1% |
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Researcher | 49 | 15% |
Student > Bachelor | 43 | 13% |
Student > Master | 40 | 13% |
Student > Doctoral Student | 13 | 4% |
Other | 38 | 12% |
Unknown | 57 | 18% |
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Agricultural and Biological Sciences | 69 | 22% |
Chemistry | 54 | 17% |
Physics and Astronomy | 22 | 7% |
Engineering | 6 | 2% |
Other | 15 | 5% |
Unknown | 61 | 19% |