Title |
A high-content imaging assay for the quantification of the Burkholderia pseudomallei induced multinucleated giant cell (MNGC) phenotype in murine macrophages
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Published in |
BMC Microbiology, April 2014
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DOI | 10.1186/1471-2180-14-98 |
Pubmed ID | |
Authors |
Gianluca Pegoraro, Brett P Eaton, Ricky L Ulrich, Douglas J Lane, Jenifer F Ojeda, Sina Bavari, David DeShazer, Rekha G Panchal |
Abstract |
Burkholderia pseudomallei (Bp), a Gram-negative, motile, facultative intracellular bacterium is the causative agent of melioidosis in humans and animals. The Bp genome encodes a repertoire of virulence factors, including the cluster 3 type III secretion system (T3SS-3), the cluster 1 type VI secretion system (T6SS-1), and the intracellular motility protein BimA, that enable the pathogen to invade both phagocytic and non-phagocytic cells. A unique hallmark of Bp infection both in vitro and in vivo is its ability to induce cell-to-cell fusion of macrophages to form multinucleated giant cells (MNGCs), which to date are semi-quantitatively reported following visual inspection. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 48 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 11 | 23% |
Student > Master | 7 | 15% |
Student > Bachelor | 6 | 13% |
Other | 5 | 10% |
Student > Doctoral Student | 4 | 8% |
Other | 5 | 10% |
Unknown | 10 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 13 | 27% |
Biochemistry, Genetics and Molecular Biology | 8 | 17% |
Immunology and Microbiology | 7 | 15% |
Nursing and Health Professions | 2 | 4% |
Unspecified | 1 | 2% |
Other | 6 | 13% |
Unknown | 11 | 23% |