↓ Skip to main content

Increased Hydrogen Production by Genetic Engineering of Escherichia coli

Overview of attention for article published in PLOS ONE, February 2009
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (85th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (64th percentile)

Mentioned by

news
1 news outlet

Citations

dimensions_citation
54 Dimensions

Readers on

mendeley
87 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Increased Hydrogen Production by Genetic Engineering of Escherichia coli
Published in
PLOS ONE, February 2009
DOI 10.1371/journal.pone.0004432
Pubmed ID
Authors

Zhanmin Fan, Ling Yuan, Ranjini Chatterjee

Abstract

Escherichia coli is capable of producing hydrogen under anaerobic growth conditions. Formate is converted to hydrogen in the fermenting cell by the formate hydrogenlyase enzyme system. The specific hydrogen yield from glucose was improved by the modification of transcriptional regulators and metabolic enzymes involved in the dissimilation of pyruvate and formate. The engineered E. coli strains ZF1 (DeltafocA; disrupted in a formate transporter gene) and ZF3 (DeltanarL; disrupted in a global transcriptional regulator gene) produced 14.9, and 14.4 micromols of hydrogen/mg of dry cell weight, respectively, compared to 9.8 micromols of hydrogen/mg of dry cell weight generated by wild-type E. coli strain W3110. The molar yield of hydrogen for strain ZF3 was 0.96 mols of hydrogen/mol of glucose, compared to 0.54 mols of hydrogen/mol of glucose for the wild-type E. coli strain. The expression of the global transcriptional regulator protein FNR at levels above natural abundance had a synergistic effect on increasing the hydrogen yield in the DeltafocA genetic background. The modification of global transcriptional regulators to modulate the expression of multiple operons required for the biosynthesis of formate hydrogenlyase represents a practical approach to improve hydrogen production.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 87 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Turkey 1 1%
France 1 1%
India 1 1%
Belgium 1 1%
United States 1 1%
Unknown 82 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 22%
Student > Master 16 18%
Researcher 13 15%
Student > Bachelor 9 10%
Student > Doctoral Student 9 10%
Other 13 15%
Unknown 8 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 42 48%
Engineering 15 17%
Biochemistry, Genetics and Molecular Biology 6 7%
Environmental Science 5 6%
Chemistry 3 3%
Other 4 5%
Unknown 12 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 July 2016.
All research outputs
#4,164,720
of 22,754,104 outputs
Outputs from PLOS ONE
#59,088
of 194,175 outputs
Outputs of similar age
#23,755
of 171,924 outputs
Outputs of similar age from PLOS ONE
#182
of 529 outputs
Altmetric has tracked 22,754,104 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 194,175 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.1. This one has gotten more attention than average, scoring higher than 68% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 171,924 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 529 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.