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Accurate single-shot quantitative phase imaging of biological specimens with telecentric digital holographic microscopy

Overview of attention for article published in Journal of Biomedical Optics, April 2014
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Title
Accurate single-shot quantitative phase imaging of biological specimens with telecentric digital holographic microscopy
Published in
Journal of Biomedical Optics, April 2014
DOI 10.1117/1.jbo.19.4.046022
Pubmed ID
Authors

Ana Doblas, Emilio Sánchez-Ortiga, Manuel Martínez-Corral, Genaro Saavedra, Jorge Garcia-Sucerquia

Abstract

The advantages of using a telecentric imaging system in digital holographic microscopy (DHM) to study biological specimens are highlighted. To this end, the performances of nontelecentric DHM and telecentric DHM are evaluated from the quantitative phase imaging (QPI) point of view. The evaluated stability of the microscope allows single-shot QPI in DHM by using telecentric imaging systems. Quantitative phase maps of a section of the head of the drosophila melanogaster fly and of red blood cells are obtained via single-shot DHM with no numerical postprocessing. With these maps we show that the use of telecentric DHM provides larger field of view for a given magnification and permits more accurate QPI measurements with less number of computational operations.

Mendeley readers

The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Korea, Republic of 1 2%
France 1 2%
Unknown 48 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 24%
Researcher 9 18%
Student > Bachelor 6 12%
Student > Doctoral Student 3 6%
Professor > Associate Professor 3 6%
Other 8 16%
Unknown 9 18%
Readers by discipline Count As %
Physics and Astronomy 22 44%
Engineering 10 20%
Biochemistry, Genetics and Molecular Biology 3 6%
Computer Science 2 4%
Mathematics 1 2%
Other 2 4%
Unknown 10 20%