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RNA Remodeling Proteins

Overview of attention for book
Cover of 'RNA Remodeling Proteins'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Key Points to Consider When Studying RNA Remodeling by Proteins
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    Chapter 2 Happy Birthday: 25 Years of DEAD-Box Proteins.
  4. Altmetric Badge
    Chapter 3 In Vivo Cross-Linking Followed by PolyA Enrichment to Identify Yeast mRNA Binding Proteins.
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    Chapter 4 Dynamics of the spb4 interactome monitored by affinity purification.
  6. Altmetric Badge
    Chapter 5 Using EMOTE to Map the Exact 5′-Ends of Processed RNA on a Transcriptome-Wide Scale
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    Chapter 6 Cellular Localization of RNA Degradation and Processing Components in Escherichia coli
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    Chapter 7 Recombineering Applications for the Mutational Analysis of Bacterial RNA-Binding Proteins and Their Sites of Action
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    Chapter 8 Determination of RNA Chaperone Activity Using an Escherichia coli Mutant
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    Chapter 9 Biochemical Characterization of G4 Quadruplex Telomerase RNA Unwinding by the RNA Helicase RHAU
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    Chapter 10 ATPase Site Configuration of the RNA Helicase DbpA Probed by ENDOR Spectroscopy
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    Chapter 11 Bioinformatics and Biochemical Methods to Study the Structural and Functional Elements of DEAD-Box RNA Helicases
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    Chapter 12 Measuring Helicase Inhibition of the DEAD-Box Protein Dbp2 by Yra1.
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    Chapter 13 A FRET-Based, Continuous Assay for the Helicase Activity of DEAD-Box Proteins
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    Chapter 14 A Fluorescence-Based Screening Assay for Identification of Hepatitis C Virus NS3 Helicase Inhibitors and Characterization of Their Inhibitory Mechanism
  16. Altmetric Badge
    Chapter 15 Mechanisms of HCV NS3 Helicase Monitored by Optical Tweezers
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    Chapter 16 Constructing a Magnetic Tweezers to Monitor RNA Translocation at the Single-Molecule Level
  18. Altmetric Badge
    Chapter 17 Probing RNA Translocases with DNA
  19. Altmetric Badge
    Chapter 18 Monitoring RNA Unwinding by the Transcription Termination Factor Rho from Mycobacterium tuberculosis
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    Chapter 19 Characterization of the mechanisms of transcription termination by the helicase sen1.
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    Chapter 20 Characterization of TRAP-Mediated Regulation of the B. subtilis trp Operon Using In Vitro Transcription and Transcriptional Reporter Fusions In Vivo
  22. Altmetric Badge
    Chapter 21 Single-Molecule FRET Characterization of RNA Remodeling Induced by an Antitermination Protein
  23. Altmetric Badge
    Chapter 22 Fluorescence Reporters for Hfq Oligomerization and RNA Annealing
  24. Altmetric Badge
    Chapter 23 Fluorescence anisotropy-based salt-titration approach to characterize protein-nucleic Acid interactions.
  25. Altmetric Badge
    Chapter 24 Probing Hfq:RNA Interactions with Hydroxyl Radical and RNase Footprinting.
  26. Altmetric Badge
    Chapter 25 Purification of Eukaryotic Exoribonucleases Following Heterologous Expression in Bacteria and Analysis of Their Biochemical Properties by In Vitro Enzymatic Assays
  27. Altmetric Badge
    Chapter 26 Tips and Tricks to Probe the RNA-Degrading Activities of Hyperthermophilic Archaeal β-CASP Ribonucleases.
Attention for Chapter 19: Characterization of the mechanisms of transcription termination by the helicase sen1.
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Chapter title
Characterization of the mechanisms of transcription termination by the helicase sen1.
Chapter number 19
Book title
RNA Remodeling Proteins
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2214-7_19
Pubmed ID
25579594
Book ISBNs
978-1-4939-2213-0, 978-1-4939-2214-7
Authors

Porrua O, Libri D, Odil Porrua, Domenico Libri

Abstract

In vitro transcription systems have been widely used to study all the steps of transcription from initiation to termination and many transcription-coupled processes. Here we describe an in vitro transcription-termination assay that we have used for the analysis of the mechanism of termination by the yeast helicase Sen1. In this system, we use highly purified proteins to assemble ternary elongation complexes (RNA polymerase, DNA template, and nascent RNA) on biotinylated DNA that is subsequently immobilized on streptavidin beads. After allowing transcription by the addition of nucleotides, the termination events can be detected and quantified by comparing the amounts of polymerases and transcripts released from the DNA templates in reactions performed in the absence or in the presence of purified Sen1. By modifying different parameters of the assay, this technique allows the study of several aspects of the termination reaction.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 24%
Professor 4 19%
Professor > Associate Professor 2 10%
Student > Ph. D. Student 2 10%
Unspecified 1 5%
Other 2 10%
Unknown 5 24%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 48%
Agricultural and Biological Sciences 3 14%
Unspecified 1 5%
Computer Science 1 5%
Medicine and Dentistry 1 5%
Other 0 0%
Unknown 5 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 January 2015.
All research outputs
#20,249,662
of 22,778,347 outputs
Outputs from Methods in molecular biology
#9,869
of 13,093 outputs
Outputs of similar age
#296,773
of 353,651 outputs
Outputs of similar age from Methods in molecular biology
#640
of 1,007 outputs
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So far Altmetric has tracked 13,093 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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