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Cardioprotection by remote ischemic preconditioning of the rat heart is mediated by extracellular vesicles

Overview of attention for article published in Journal of Molecular & Cellular Cardiology, March 2014
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#8 of 404)
  • High Attention Score compared to outputs of the same age (88th percentile)
  • High Attention Score compared to outputs of the same age and source (87th percentile)

Mentioned by

news
1 news outlet

Citations

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149 Dimensions

Readers on

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149 Mendeley
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Title
Cardioprotection by remote ischemic preconditioning of the rat heart is mediated by extracellular vesicles
Published in
Journal of Molecular & Cellular Cardiology, March 2014
DOI 10.1016/j.yjmcc.2014.01.004
Pubmed ID
Authors

Zoltán Giricz, Zoltán V. Varga, Tamás Baranyai, Péter Sipos, Krisztina Pálóczi, Ágnes Kittel, Edit I. Buzás, Péter Ferdinandy

Abstract

Remote ischemic preconditioning (RIPC) of the heart is exerted by brief ischemic insults affected on a remote organ or a remote area of the heart before a sustained cardiac ischemia. To date, little is known about the inter-organ transfer mechanisms of cardioprotection by RIPC. Exosomes and microvesicles/microparticles are vesicles of 30-100 nm and 100-1000 nm in diameter, respectively (collectively termed extracellular vesicles [EVs]). Their content of proteins, mRNAs and microRNAs, renders EV ideal conveyors of inter-organ communication. However, whether EVs are involved in RIPC, is unknown. Therefore, here we investigated whether (1) IPC induces release of EVs from the heart, and (2) EVs are necessary for cardioprotection by RIPC. Hearts of male Wistar rats were isolated and perfused in Langendorff mode. A group of donor hearts was exposed to 3 × 5-5 min global ischemia and reperfusion (IPC) or 30 min aerobic perfusion, while coronary perfusates were collected. Coronary perfusates of these hearts were given to another set of recipient isolated hearts. A group of recipient hearts received IPC effluent depleted of EVs by differential ultracentrifugation. Infarct size was determined after 30 min global ischemia and 120 min reperfusion. The presence or absence of EVs in perfusates was confirmed by dynamic light scattering, the EV marker HSP60 Western blot, and electron microscopy. IPC markedly increased EV release from the heart as assessed by HSP60. Administration of coronary perfusate from IPC donor hearts attenuated infarct size in non-preconditioned recipient hearts (12.9 ± 1.6% vs. 25.0 ± 2.7%), similarly to cardioprotection afforded by IPC (7.3 ± 2.7% vs. 22.1 ± 2.9%) on the donor hearts. Perfusates of IPC hearts depleted of EVs failed to exert cardioprotection in recipient hearts (22.0 ± 2.3%). This is the first demonstration that EVs released from the heart after IPC are necessary for cardioprotection by RIPC, evidencing the importance of vesicular transfer mechanisms in remote cardioprotection.

Mendeley readers

The data shown below were compiled from readership statistics for 149 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 1%
Brazil 1 <1%
Chile 1 <1%
Canada 1 <1%
Japan 1 <1%
Netherlands 1 <1%
Unknown 142 95%

Demographic breakdown

Readers by professional status Count As %
Researcher 33 22%
Student > Ph. D. Student 32 21%
Student > Bachelor 16 11%
Student > Master 16 11%
Student > Doctoral Student 15 10%
Other 23 15%
Unknown 14 9%
Readers by discipline Count As %
Medicine and Dentistry 58 39%
Agricultural and Biological Sciences 34 23%
Biochemistry, Genetics and Molecular Biology 19 13%
Neuroscience 5 3%
Engineering 3 2%
Other 9 6%
Unknown 21 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 May 2014.
All research outputs
#301,243
of 3,746,945 outputs
Outputs from Journal of Molecular & Cellular Cardiology
#8
of 404 outputs
Outputs of similar age
#10,436
of 95,864 outputs
Outputs of similar age from Journal of Molecular & Cellular Cardiology
#1
of 8 outputs
Altmetric has tracked 3,746,945 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 404 research outputs from this source. They receive a mean Attention Score of 2.1. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 95,864 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them