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The ATRX cDNA is prone to bacterial IS10 element insertions that alter its structure

Overview of attention for article published in SpringerPlus, May 2014
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Title
The ATRX cDNA is prone to bacterial IS10 element insertions that alter its structure
Published in
SpringerPlus, May 2014
DOI 10.1186/2193-1801-3-222
Pubmed ID
Abstract

The SWI/SNF-like chromatin-remodeling protein ATRX has emerged as a key factor in the regulation of α-globin gene expression, incorporation of histone variants into the chromatin template and, more recently, as a frequently mutated gene across a wide spectrum of cancers. Therefore, the availability of a functional ATRX cDNA for expression studies is a valuable tool for the scientific community. We have identified two independent transposon insertions of a bacterial IS10 element into exon 8 of ATRX isoform 2 coding sequence in two different plasmids derived from a single source. We demonstrate that these insertion events are common and there is an insertion hotspot within the ATRX cDNA. Such IS10 insertions produce a truncated form of ATRX, which significantly compromises its nuclear localization. In turn, we describe ways to prevent IS10 insertion during propagation and cloning of ATRX-containing vectors, including optimal growth conditions, bacterial strains, and suggested sequencing strategies. Finally, we have generated an insertion-free plasmid that is available to the community for expression studies of ATRX.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Mexico 1 8%
Unknown 11 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 33%
Student > Ph. D. Student 3 25%
Professor 1 8%
Student > Bachelor 1 8%
Student > Master 1 8%
Other 2 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 42%
Agricultural and Biological Sciences 4 33%
Pharmacology, Toxicology and Pharmaceutical Science 1 8%
Immunology and Microbiology 1 8%
Unknown 1 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 May 2014.
All research outputs
#2,088,851
of 3,746,941 outputs
Outputs from SpringerPlus
#391
of 750 outputs
Outputs of similar age
#49,974
of 95,862 outputs
Outputs of similar age from SpringerPlus
#39
of 52 outputs
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