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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Alternative Splicing and Highly Variable Cadherin Transcripts Associated with Field-Evolved Resistance of Pink Bollworm to Bt Cotton in India
|
|---|---|
| Published in |
PLOS ONE, May 2014
|
| DOI | 10.1371/journal.pone.0097900 |
| Pubmed ID | |
| Authors |
Jeffrey A. Fabrick, Jeyakumar Ponnuraj, Amar Singh, Raj K. Tanwar, Gopalan C. Unnithan, Alex J. Yelich, Xianchun Li, Yves Carrière, Bruce E. Tabashnik |
| Abstract |
Evolution of resistance by insect pests can reduce the benefits of insecticidal proteins from Bacillus thuringiensis (Bt) that are used extensively in sprays and transgenic crops. Despite considerable knowledge of the genes conferring insect resistance to Bt toxins in laboratory-selected strains and in field populations exposed to Bt sprays, understanding of the genetic basis of field-evolved resistance to Bt crops remains limited. In particular, previous work has not identified the genes conferring resistance in any cases where field-evolved resistance has reduced the efficacy of a Bt crop. Here we report that mutations in a gene encoding a cadherin protein that binds Bt toxin Cry1Ac are associated with field-evolved resistance of pink bollworm (Pectinophora gossypiella) in India to Cry1Ac produced by transgenic cotton. We conducted laboratory bioassays that confirmed previously reported resistance to Cry1Ac in pink bollworm from the state of Gujarat, where Bt cotton producing Cry1Ac has been grown extensively. Analysis of DNA from 436 pink bollworm from seven populations in India detected none of the four cadherin resistance alleles previously reported to be linked with resistance to Cry1Ac in laboratory-selected strains of pink bollworm from Arizona. However, DNA sequencing of pink bollworm derived from resistant and susceptible field populations in India revealed eight novel, severely disrupted cadherin alleles associated with resistance to Cry1Ac. For these eight alleles, analysis of complementary DNA (cDNA) revealed a total of 19 transcript isoforms, each containing a premature stop codon, a deletion of at least 99 base pairs, or both. Seven of the eight disrupted alleles each produced two or more different transcript isoforms, which implicates alternative splicing of messenger RNA (mRNA). This represents the first example of alternative splicing associated with field-evolved resistance that reduced the efficacy of a Bt crop. |
X Demographics
The data shown below were collected from the profiles of 23 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 17% |
| India | 3 | 13% |
| Chile | 2 | 9% |
| Paraguay | 1 | 4% |
| Australia | 1 | 4% |
| France | 1 | 4% |
| Unknown | 11 | 48% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 17 | 74% |
| Scientists | 5 | 22% |
| Science communicators (journalists, bloggers, editors) | 1 | 4% |
Mendeley readers
The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 1% |
| United States | 1 | 1% |
| Peru | 1 | 1% |
| Unknown | 69 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 17 | 24% |
| Student > Ph. D. Student | 10 | 14% |
| Student > Master | 10 | 14% |
| Student > Doctoral Student | 5 | 7% |
| Student > Bachelor | 5 | 7% |
| Other | 9 | 13% |
| Unknown | 16 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 33 | 46% |
| Biochemistry, Genetics and Molecular Biology | 13 | 18% |
| Environmental Science | 6 | 8% |
| Nursing and Health Professions | 1 | 1% |
| Medicine and Dentistry | 1 | 1% |
| Other | 1 | 1% |
| Unknown | 17 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 50. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 May 2018.
All research outputs
#708,861
of 22,756,196 outputs
Outputs from PLOS ONE
#9,938
of 194,180 outputs
Outputs of similar age
#7,410
of 227,120 outputs
Outputs of similar age from PLOS ONE
#252
of 4,727 outputs
Altmetric has tracked 22,756,196 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 194,180 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.1. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 227,120 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 4,727 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.