Chapter title |
Extraction of plant secondary metabolites.
|
---|---|
Chapter number | 13 |
Book title |
Natural Products Isolation
|
Published in |
Methods in molecular biology, January 2012
|
DOI | 10.1007/978-1-61779-624-1_13 |
Pubmed ID | |
Book ISBNs |
978-1-61779-623-4, 978-1-61779-624-1
|
Authors |
William P Jones, A Douglas Kinghorn, William P. Jones, A. Douglas Kinghorn, Jones, William P., Kinghorn, A. Douglas |
Abstract |
This chapter presents an overview of the preparation of extracts from plants using organic solvents, with emphasis on common problems encountered and methods for their reduction or elimination. In addition to generally applicable extraction protocols, methods are suggested for selectively extracting specific classes of plant-derived compounds, and phytochemical procedures are presented for the detection of classes of compounds encountered commonly during extraction, including selected groups of secondary metabolites and interfering compounds. Successful extraction begins with careful selection and preparation of plant samples and thorough review of the appropriate literature for suitable protocols for a particular class of compounds or plant species. During the extraction of plant material, it is important to minimize interference from compounds that may co-extract with the target compounds, and to avoid contamination of the extract, as well as to prevent decomposition of important metabolites or artifact formation as a result of extraction conditions or solvent impurities. |
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United States | 2 | <1% |
India | 2 | <1% |
South Africa | 1 | <1% |
Germany | 1 | <1% |
French Polynesia | 1 | <1% |
Mexico | 1 | <1% |
Peru | 1 | <1% |
Luxembourg | 1 | <1% |
Other | 1 | <1% |
Unknown | 620 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 93 | 15% |
Student > Ph. D. Student | 86 | 14% |
Student > Master | 67 | 11% |
Researcher | 50 | 8% |
Lecturer | 25 | 4% |
Other | 88 | 14% |
Unknown | 226 | 36% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 142 | 22% |
Chemistry | 79 | 12% |
Biochemistry, Genetics and Molecular Biology | 62 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 47 | 7% |
Medicine and Dentistry | 23 | 4% |
Other | 46 | 7% |
Unknown | 236 | 37% |