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Metabolomic Profiling and Genomic Study of a Marine Sponge-Associated Streptomyces sp.

Overview of attention for article published in Marine Drugs, June 2014
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (56th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (56th percentile)

Mentioned by

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2 tweeters
facebook
1 Facebook page

Citations

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39 Dimensions

Readers on

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112 Mendeley
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Title
Metabolomic Profiling and Genomic Study of a Marine Sponge-Associated Streptomyces sp.
Published in
Marine Drugs, June 2014
DOI 10.3390/md12063323
Pubmed ID
Authors

Christina Viegelmann, Lekha Margassery, Jonathan Kennedy, Tong Zhang, Ciarán O'Brien, Fergal O'Gara, John Morrissey, Alan Dobson, RuAngelie Edrada-Ebel

Abstract

Metabolomics and genomics are two complementary platforms for analyzing an organism as they provide information on the phenotype and genotype, respectively. These two techniques were applied in the dereplication and identification of bioactive compounds from a Streptomyces sp. (SM8) isolated from the sponge Haliclona simulans from Irish waters. Streptomyces strain SM8 extracts showed antibacterial and antifungal activity. NMR analysis of the active fractions proved that hydroxylated saturated fatty acids were the major components present in the antibacterial fractions. Antimycin compounds were initially putatively identified in the antifungal fractions using LC-Orbitrap. Their presence was later confirmed by comparison to a standard. Genomic analysis of Streptomyces sp. SM8 revealed the presence of multiple secondary metabolism gene clusters, including a gene cluster for the biosynthesis of the antifungal antimycin family of compounds. The antimycin gene cluster of Streptomyces sp. SM8 was inactivated by disruption of the antimycin biosynthesis gene antC. Extracts from this mutant strain showed loss of antimycin production and significantly less antifungal activity than the wild-type strain. Three butenolides, 4,10-dihydroxy-10-methyl-dodec-2-en-1,4-olide (1), 4,11-dihydroxy-10-methyl-dodec-2-en-1,4-olide (2), and 4-hydroxy-10-methyl-11-oxo-dodec-2-en-1,4-olide (3) that had previously been reported from marine Streptomyces species were also isolated from SM8. Comparison of the extracts of Streptomyces strain SM8 and its host sponge, H. simulans, using LC-Orbitrap revealed the presence of metabolites common to both extracts, providing direct evidence linking sponge metabolites to a specific microbial symbiont.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 112 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 2 2%
United Kingdom 1 <1%
Germany 1 <1%
Spain 1 <1%
Philippines 1 <1%
Unknown 106 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 23 21%
Student > Doctoral Student 14 13%
Student > Master 10 9%
Lecturer 10 9%
Student > Bachelor 10 9%
Other 31 28%
Unknown 14 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 33 29%
Chemistry 21 19%
Biochemistry, Genetics and Molecular Biology 12 11%
Pharmacology, Toxicology and Pharmaceutical Science 7 6%
Immunology and Microbiology 6 5%
Other 12 11%
Unknown 21 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 July 2014.
All research outputs
#7,065,970
of 12,520,143 outputs
Outputs from Marine Drugs
#666
of 1,564 outputs
Outputs of similar age
#79,065
of 189,404 outputs
Outputs of similar age from Marine Drugs
#14
of 41 outputs
Altmetric has tracked 12,520,143 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,564 research outputs from this source. They receive a mean Attention Score of 3.7. This one has gotten more attention than average, scoring higher than 56% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 189,404 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.
We're also able to compare this research output to 41 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.