Title |
Characterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level
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Published in |
BMC Microbiology, May 2014
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DOI | 10.1186/1471-2180-14-138 |
Pubmed ID | |
Authors |
Glaucia EO Midorikawa, Maria de Lourdes M de Sousa, Otniel Freitas Silva, Jurema do Socorro A Dias, Luis IB Kanzaki, Rogerio E Hanada, Renata MLC Mesquita, Rivadalve C Gonçalves, Virginia S Alvares, Daniela MC Bittencourt, Robert NG Miller |
Abstract |
Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Venezuela, Bolivarian Republic of | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Brazil | 1 | 2% |
Unknown | 50 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 9 | 18% |
Researcher | 9 | 18% |
Student > Ph. D. Student | 6 | 12% |
Student > Bachelor | 6 | 12% |
Student > Doctoral Student | 5 | 10% |
Other | 10 | 20% |
Unknown | 6 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 23 | 45% |
Chemistry | 3 | 6% |
Immunology and Microbiology | 2 | 4% |
Engineering | 2 | 4% |
Biochemistry, Genetics and Molecular Biology | 2 | 4% |
Other | 9 | 18% |
Unknown | 10 | 20% |