| Chapter title |
Estimation of Photorespiratory Fluxes by Gas Exchange
|
|---|---|
| Chapter number | 1 |
| Book title |
Photorespiration
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7225-8_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7224-1, 978-1-4939-7225-8
|
| Authors |
Florian A. Busch, Ross M. Deans, Meisha-Marika Holloway-Phillips |
| Abstract |
Photorespiratory fluxes can be easily estimated by photosynthetic gas exchange using an infrared gas analyzer and applying the Farquhar, von Caemmerer, and Berry (Farquhar et al. Planta 149:78-90, 1980) photosynthesis model. For a more direct measurement of photorespiratory CO2 release from glycine decarboxylation, infrared gas analysis can be coupled to membrane-inlet mass spectrometry, capable of separating the total CO2 concentration into its (12)CO2 and (13)CO2 components in a continuous online fashion. This chapter discusses how to calculate rates of photorespiration from Rubisco kinetics and describes in detail a method for measuring the CO2 release from glycine decarboxylation using (13)CO2. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 33% |
| Student > Master | 1 | 33% |
| Unknown | 1 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 67% |
| Unknown | 1 | 33% |