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Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases

Overview of attention for article published in Microbial Cell Factories, June 2017
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Title
Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases
Published in
Microbial Cell Factories, June 2017
DOI 10.1186/s12934-017-0723-8
Pubmed ID
Authors

Yao Zhang, Xiao Luan, Huaiyuan Zhang, Victoriano Garre, Yuanda Song, Colin Ratledge

Abstract

γ-Linolenic acid (GLA) is important because of its nutritional value and medicinal applications. Although the biosynthetic pathways of some plant and microbial GLA have been deciphered, current understanding of the correlation between desaturases and GLA synthesis in oleaginous fungi is incomplete. In previous work, we found that a large amount of oleic acid (OA) had not been converted to linoleic acid (LA) or GLA in Mucor circinelloides CBS 277.49, which may be due to inadequate activities of the delta-12 or delta-6 desaturases, and thus leading to the accumulation of OA and LA. Thus, it is necessary to explore the main contributing factor during the process of GLA biosynthesis in M. circinelloides. To enhance GLA production in M. circinelloides, homologous overexpression of delta-12 and two delta-6 desaturases (named delta-6-1 and delta-6-2, respectively) were analyzed. When delta-6 desaturase were overexpressed in M. circinelloides, up to 43% GLA was produced in the total fatty acids, and the yield of GLA reached 180 mg/l, which were, respectively, 38 and 33% higher than the control strain. These findings revealed that delta-6 desaturase (especially for delta-6-1 desaturase) plays an important role in GLA synthesis by M. circinelloides. The strain overexpressing delta-6-1 desaturase may have potential application in microbial GLA production.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
China 1 10%
Unknown 9 90%

Demographic breakdown

Readers by professional status Count As %
Unspecified 4 40%
Student > Ph. D. Student 2 20%
Researcher 2 20%
Student > Bachelor 1 10%
Student > Doctoral Student 1 10%
Other 0 0%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 50%
Unspecified 4 40%
Agricultural and Biological Sciences 1 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 September 2017.
All research outputs
#9,344,127
of 11,689,928 outputs
Outputs from Microbial Cell Factories
#601
of 845 outputs
Outputs of similar age
#192,963
of 263,738 outputs
Outputs of similar age from Microbial Cell Factories
#9
of 11 outputs
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We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.