Title |
Sample-ready multiplex qPCR assay for detection of malaria
|
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Published in |
Malaria Journal, April 2014
|
DOI | 10.1186/1475-2875-13-158 |
Pubmed ID | |
Authors |
Edwin Kamau, Saba Alemayehu, Karla C Feghali, Dennis W Juma, George M Blackstone, William R Marion, Peter Obare, Bernhards Ogutu, Christian F Ockenhouse |
Abstract |
Microscopy and antigen detecting rapid diagnostic tests are the diagnostic tests of choice in management of clinical malaria. However, due to their limitations, the need to utilize more sensitive methods such as real-time PCR (qPCR) is evident as more studies are now utilizing molecular methods in detection of malaria. Some of the challenges that continue to limit the widespread utilization of qPCR include lack of assay standardization, assay variability, risk of contamination, and the need for cold-chain. Lyophilization of molecular assays can overcome some of these limitations and potentially enable widespread qPCR utilization. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 1% |
Hungary | 1 | 1% |
Greece | 1 | 1% |
Portugal | 1 | 1% |
Unknown | 85 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 18 | 20% |
Researcher | 17 | 19% |
Student > Master | 14 | 16% |
Student > Bachelor | 9 | 10% |
Student > Doctoral Student | 6 | 7% |
Other | 8 | 9% |
Unknown | 17 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 24 | 27% |
Engineering | 10 | 11% |
Medicine and Dentistry | 10 | 11% |
Biochemistry, Genetics and Molecular Biology | 8 | 9% |
Immunology and Microbiology | 4 | 4% |
Other | 13 | 15% |
Unknown | 20 | 22% |