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Epigenetic regulation of inflammation in localized aggressive periodontitis

Overview of attention for article published in Clinical Epigenetics, September 2017
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Title
Epigenetic regulation of inflammation in localized aggressive periodontitis
Published in
Clinical Epigenetics, September 2017
DOI 10.1186/s13148-017-0385-8
Pubmed ID
Authors

L. M. Shaddox, A. F. Mullersman, H. Huang, S. M. Wallet, T. Langaee, I. Aukhil

Abstract

We have previously demonstrated a Toll-like receptor (TLR)-mediated hyper-responsive phenotype in our cohort of localized aggressive periodontitis (LAP) individuals. However, mechanisms related to this phenotype are still not clear in the literature. The objective of this cross-sectional study is to examine the role of epigenetic regulation, specifically DNA methylation status of genes in the TLR pathway in this cohort. Peripheral blood was collected from 20 LAP patients and 20 healthy unrelated controls. Whole blood was stimulated with 1 μl (100 ng/μl) of purified Escherichia coli lipopolysaccharide (LPS) for 24 h and cyto/chemokines in the supernatants analyzed by Luminex multiplex assays. Genomic DNA extracted from buffy coats prepared from a second tube of whole blood was used for DNA methylation analysis by pyrosequencing of seven TLR signaling genes (FADD, MAP3K7, MYD88, IL6R, PPARA, IRAK1BP1, RIPK2). Significant differences in the methylation status were observed at specific CpG positions in LAP patients compared to healthy controls and interestingly also between severe and moderate LAP. Specifically, subjects with moderate LAP presented hypermethylation of both the upregulating (MAP3K7, MYD88, IL6R, and RIPK2) and downregulating (FADD, IRAK, and PPARA) genes, while severe LAP presented hypomethylation of these genes. Further analysis on CpG sites with significant differences in methylation status correlates with an increased pro-inflammatory cytokine profile for LAP patients. Our findings suggest that epigenetic modifications of genes in the TLR pathway may orchestrate the thresholds for balancing induction and prevention of tissue destruction during the course of disease, and thus differ significantly at different stages of the disease, where moderate LAP shows hypermethylation and severe LAP shows hypomethylation of several genes. https://clinicaltrials.gov, NCT01330719.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 27 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 26%
Researcher 3 11%
Student > Ph. D. Student 3 11%
Unspecified 2 7%
Student > Bachelor 2 7%
Other 4 15%
Unknown 6 22%
Readers by discipline Count As %
Medicine and Dentistry 10 37%
Biochemistry, Genetics and Molecular Biology 4 15%
Unspecified 2 7%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Agricultural and Biological Sciences 1 4%
Other 2 7%
Unknown 7 26%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 September 2017.
All research outputs
#10,366,087
of 11,691,744 outputs
Outputs from Clinical Epigenetics
#495
of 542 outputs
Outputs of similar age
#222,657
of 263,836 outputs
Outputs of similar age from Clinical Epigenetics
#18
of 33 outputs
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