Title |
Characterization of the vaginal microbiota of healthy Canadian women through the menstrual cycle
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Published in |
Microbiome, July 2014
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DOI | 10.1186/2049-2618-2-23 |
Pubmed ID | |
Authors |
Bonnie Chaban, Matthew G Links, Teenus Paramel Jayaprakash, Emily C Wagner, Danielle K Bourque, Zoe Lohn, Arianne YK Albert, Julie van Schalkwyk, Gregor Reid, Sean M Hemmingsen, Janet E Hill, Deborah M Money |
Abstract |
The vaginal microbial community plays a vital role in maintaining women's health. Understanding the precise bacterial composition is challenging because of the diverse and difficult-to-culture nature of many bacterial constituents, necessitating culture-independent methodology. During a natural menstrual cycle, physiological changes could have an impact on bacterial growth, colonization, and community structure. The objective of this study was to assess the stability of the vaginal microbiome of healthy Canadian women throughout a menstrual cycle by using cpn60-based microbiota analysis. Vaginal swabs from 27 naturally cycling reproductive-age women were collected weekly through a single menstrual cycle. Polymerase chain reaction (PCR) was performed to amplify the universal target region of the cpn60 gene and generate amplicons representative of the microbial community. Amplicons were pyrosequenced, assembled into operational taxonomic units, and analyzed. Samples were also assayed for total 16S rRNA gene content and Gardnerella vaginalis by quantitative PCR and screened for the presence of Mollicutes by using family and genus-specific PCR. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 50% |
Venezuela, Bolivarian Republic of | 1 | 13% |
India | 1 | 13% |
Unknown | 2 | 25% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 6 | 75% |
Scientists | 2 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 1% |
Italy | 1 | <1% |
South Africa | 1 | <1% |
Brazil | 1 | <1% |
Peru | 1 | <1% |
Canada | 1 | <1% |
Unknown | 291 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 49 | 16% |
Student > Ph. D. Student | 48 | 16% |
Student > Master | 46 | 15% |
Student > Bachelor | 35 | 12% |
Student > Doctoral Student | 20 | 7% |
Other | 47 | 16% |
Unknown | 55 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 71 | 24% |
Medicine and Dentistry | 50 | 17% |
Biochemistry, Genetics and Molecular Biology | 49 | 16% |
Immunology and Microbiology | 30 | 10% |
Nursing and Health Professions | 4 | 1% |
Other | 25 | 8% |
Unknown | 71 | 24% |