Title |
Evaluation of Xpert MTB/RIF testing for rapid diagnosis of childhood pulmonary tuberculosis in children by Xpert MTB/RIF testing of stool samples in a low resource setting
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Published in |
BMC Research Notes, September 2017
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DOI | 10.1186/s13104-017-2806-3 |
Pubmed ID | |
Authors |
Zahra Hasan, Sadia Shakoor, Fehmina Arif, Aisha Mehnaz, Alnoor Akber, Marium Haider, Akbar Kanji, Rumina Hasan |
Abstract |
Children with tuberculosis (TB) remain underdiagnosed due to difficulty in testing for Mycobacterium tuberculosis (MTB) infection. We evaluated the Xpert MTB/RIF assay for respiratory and stool testing in children for pulmonary TB through a cross-sectional study at tertiary care facilities in Karachi, Pakistan. Fifty children aged 0-15 years screened by a modified Kenneth-Jones (KJ) score were included. Mycobacterial culture of respiratory samples was the microbiological standard against stool Xpert TB results. All positive TB cases were compared against a treatment response standard (TRS). Twelve study subjects were diagnosed by Xpert TB and nine by MTB culture. Compared with culture [gastric aspirates (GA)/sputum (spm)], stool Xpert TB had a sensitivity of 88.9% (95% CI 50.7-99.4) and a specificity of 95% (95% CI 81.8-99.1). Xpert TB stool versus GA/spm had sensitivity of 81.8% (95% CI 47.8-96.8) and specificity of 94.7% (95% CI 84.6-99.9). We found good agreement (kappa scores of >0.8) between stool Xpert, GA/spm Xpert and GA/spm culture. Stool Xpert PPV and NPV against TRS was 100 and 82.1% respectively. Stool Xpert TB is a relatively easy option for diagnosis for pulmonary childhood TB in a high burden low-resource setting. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 76 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 15 | 20% |
Student > Master | 12 | 16% |
Other | 7 | 9% |
Student > Postgraduate | 6 | 8% |
Student > Doctoral Student | 5 | 7% |
Other | 9 | 12% |
Unknown | 22 | 29% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 36 | 47% |
Immunology and Microbiology | 7 | 9% |
Nursing and Health Professions | 3 | 4% |
Agricultural and Biological Sciences | 2 | 3% |
Energy | 1 | 1% |
Other | 1 | 1% |
Unknown | 26 | 34% |