Title |
Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample
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Published in |
BMC Biotechnology, July 2014
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DOI | 10.1186/1472-6750-14-63 |
Pubmed ID | |
Authors |
Raffaele Altara, Marco Manca, Marleen HM Hessel, Ben J Janssen, Harry H A Struijker-Boudier, Rob JJ Hermans, W Matthijs Blankesteijn |
Abstract |
Inflammatory mediators can serve as biomarkers for the monitoring of the disease progression or prognosis in many conditions. In the present study we introduce an adaptation of a membrane-based technique in which the level of up to 40 cytokines and chemokines can be determined in both human and rodent blood in a semi-quantitative way. The planar assay was modified using the LI-COR (R) detection system (fluorescence based) rather than chemiluminescence and semi-quantitative outcomes were achieved by normalizing the outcomes using the automated exposure settings of the Odyssey readout device. The results were compared to the gold standard assay, namely ELISA. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 19 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 6 | 32% |
Student > Ph. D. Student | 4 | 21% |
Student > Bachelor | 3 | 16% |
Professor | 1 | 5% |
Other | 1 | 5% |
Other | 2 | 11% |
Unknown | 2 | 11% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 4 | 21% |
Agricultural and Biological Sciences | 3 | 16% |
Engineering | 2 | 11% |
Medicine and Dentistry | 2 | 11% |
Chemical Engineering | 1 | 5% |
Other | 3 | 16% |
Unknown | 4 | 21% |