Title |
Genetic transformation of Fusarium avenaceum by Agrobacterium tumefaciens mediated transformation and the development of a USER-Brick vector construction system
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Published in |
BMC Molecular and Cell Biology, July 2014
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DOI | 10.1186/1471-2199-15-15 |
Pubmed ID | |
Authors |
Lisette Quaade Sørensen, Erik Lysøe, Jesper Erup Larsen, Paiman Khorsand-Jamal, Kristian Fog Nielsen, Rasmus John Normand Frandsen |
Abstract |
The plant pathogenic and saprophytic fungus Fusarium avenaceum causes considerable in-field and post-field losses worldwide due to its infections of a wide range of different crops. Despite its significant impact on the profitability of agriculture production and a desire to characterize the infection process at the molecular biological level, no genetic transformation protocol has yet been established for F. avenaceum. In the current study, it is shown that F. avenaceum can be efficiently transformed by Agrobacterium tumefaciens mediated transformation. In addition, an efficient and versatile single step vector construction strategy relying on Uracil Specific Excision Reagent (USER) Fusion cloning, is developed. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Venezuela, Bolivarian Republic of | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 1 | 33% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | 1% |
Unknown | 80 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 22 | 27% |
Student > Bachelor | 13 | 16% |
Researcher | 11 | 14% |
Student > Master | 11 | 14% |
Student > Doctoral Student | 5 | 6% |
Other | 10 | 12% |
Unknown | 9 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 47 | 58% |
Biochemistry, Genetics and Molecular Biology | 9 | 11% |
Chemistry | 4 | 5% |
Engineering | 3 | 4% |
Medicine and Dentistry | 2 | 2% |
Other | 2 | 2% |
Unknown | 14 | 17% |