Title |
A Novel Role for hSMG-1 in Stress Granule Formation
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Published in |
Molecular & Cellular Biology, March 2023
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DOI | 10.1128/mcb.05987-11 |
Pubmed ID | |
Authors |
James A. L. Brown, Tara L. Roberts, Renee Richards, Rick Woods, Geoff Birrell, Y. C. Lim, Shigeo Ohno, Akio Yamashita, Robert T. Abraham, Nuri Gueven, Martin F. Lavin |
Abstract |
hSMG-1 is a member of the phosphoinositide 3 kinase-like kinase (PIKK) family with established roles in nonsense-mediated decay (NMD) of mRNA containing premature termination codons and in genotoxic stress responses to DNA damage. We report here a novel role for hSMG-1 in cytoplasmic stress granule (SG) formation. Exposure of cells to stress causing agents led to the localization of hSMG-1 to SG, identified by colocalization with TIA-1, G3BP1, and eIF4G. hSMG-1 small interfering RNA and the PIKK inhibitor wortmannin prevented formation of a subset of SG, while specific inhibitors of ATM, DNA-PK(cs), or mTOR had no effect. Exposure of cells to H(2)O(2) and sodium arsenite induced (S/T)Q phosphorylation of proteins. While Upf2 and Upf1, an essential substrate for hSMG-1 in NMD, were present in SG, NMD-specific Upf1 phosphorylation was not detected in SG, indicating hSMG-1's role in SG is separate from classical NMD. Thus, SG formation appears more complex than originally envisaged and hSMG-1 plays a central role in this process. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Japan | 2 | 2% |
United States | 1 | 1% |
Canada | 1 | 1% |
Switzerland | 1 | 1% |
Unknown | 83 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 21 | 24% |
Student > Ph. D. Student | 20 | 23% |
Student > Master | 10 | 11% |
Professor | 7 | 8% |
Student > Doctoral Student | 6 | 7% |
Other | 9 | 10% |
Unknown | 15 | 17% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 27 | 31% |
Neuroscience | 4 | 5% |
Philosophy | 2 | 2% |
Medicine and Dentistry | 2 | 2% |
Other | 1 | 1% |
Unknown | 17 | 19% |