Chapter title |
Minor histocompatibility antigen typing by DNA sequencing for clinical practice in hematopoietic stem-cell transplantation.
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Chapter number | 29 |
Book title |
Immunogenetics
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Published in |
Methods in molecular biology, January 2012
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DOI | 10.1007/978-1-61779-842-9_29 |
Pubmed ID | |
Book ISBNs |
978-1-61779-841-2, 978-1-61779-842-9
|
Authors |
Eric Spierings, Els Goulmy, Spierings, Eric, Goulmy, Els |
Abstract |
In HLA-matched stem-cell transplantation (SCT), minor H antigens are key molecules driving allo-immune responses in both graft-versus-host disease (GvHD) and in graft-versus-leukemia (GvL) reactivity. Dissection of the dual function of minor H antigens became evident through their different modes of tissue and cell expression, i.e., hematopoietic system restricted or broad. Broadly expressed minor H antigens are the targets of immune responses in both arms of graft-versus-host (GvH) responses, i.e., both GvHD and GvL, whereas the immune responses against the hematopoietic system-specific minor H antigens are restricted to the GvL arm of SCT. Evidently, it is this latter group of minor H antigens that can function as curative tools for stem-cell (SC)-based immunotherapy of hematological malignancies and disorders. The HLA-matched patient/donor combinations, incompatible for one of the hematopoietic-specific minor H antigens, are suitable for minor H antigen immunotherapy (Goulmy, Immunol Rev 157:125-140, 1997). Information on the minor H antigen phenotype is therefore needed. Hereto, genomic typing for minor H antigens has been implemented in many HLA laboratories. Here, we firstly summarize the relevance of minor H antigens particularly in hematopoietic SCT. Secondly, we describe a method for typing the various polymorphic minor H antigens molecularly identified to date by DNA sequencing. |
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