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Characteristics of influenza H13N8 subtype virus firstly isolated from Qinghai Lake Region, China

Overview of attention for article published in Virology Journal, September 2017
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Title
Characteristics of influenza H13N8 subtype virus firstly isolated from Qinghai Lake Region, China
Published in
Virology Journal, September 2017
DOI 10.1186/s12985-017-0842-1
Pubmed ID
Authors

Jie Dong, Hong Bo, Ye Zhang, Libo Dong, Shumei Zou, Weijuan Huang, Jia Liu, Dayan Wang, Yuelong Shu

Abstract

Since the highly pathogenic H5N1 influenza caused thousands of deaths of wild bird in this area in 2005, Qinghai Lake in China has become a hot spot for study of the influence of avian influenza to migratory wild birds. However, the ecology and evolution of low pathogenic avian influenza virus in this region are limited. This project-based avian influenza surveillance in Qinghai lake region was initiated in year 2012. Samples of wild bird feces and lake surface water were collected in Qinghai Lake in year 2012.Virus isolation was conducted on embryonated chicken eggs. The influenza A virus was determined by rRT-PCR. Virus sequences were acquired by deep sequencing. The phylogenetic correlation and molecular characteristics of the viruses were analyzed. The virus growth and infection features, receptor binding preference were studied, and pathogenicity in vitro as well as. Two H13N8 subtype influenza viruses were isolated. The viruses are phylogenetically belong to Eurasian lineage. Most of the genes are associated with gull origin influenza virus except PB1 gene, which is most probably derived from Anseriformes virus. The evidence of interspecies reassortment was presented. The two viruses have limited growth capacity on MDCK and A549 cells while grow well in embryonated eggs. The dual receptor binding features of the two viruses was shown up. The low pathogenic features were determined by trypsin dependence plaque formation assay. The two H13N8 subtype influenza viruses are highly associated with gull origin. The interspecies reassortment of H13 subtype virus among Anseriforme sand Charadriiformes wild birds emphasizes the importance of strengthening avian influenza surveillance in this region. This study is helpful to understand the ecology, evolution and transmission pattern of H13 subtype influenza virus globally.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 31%
Student > Bachelor 3 23%
Student > Master 2 15%
Professor 1 8%
Professor > Associate Professor 1 8%
Other 0 0%
Unknown 2 15%
Readers by discipline Count As %
Medicine and Dentistry 3 23%
Agricultural and Biological Sciences 3 23%
Veterinary Science and Veterinary Medicine 2 15%
Immunology and Microbiology 1 8%
Biochemistry, Genetics and Molecular Biology 1 8%
Other 0 0%
Unknown 3 23%