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Zika detection: comparison of methodologies

Overview of attention for article published in Brazilian Journal of Microbiology, January 2018
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1 tweeter

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98 Mendeley
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Title
Zika detection: comparison of methodologies
Published in
Brazilian Journal of Microbiology, January 2018
DOI 10.1016/j.bjm.2017.04.011
Pubmed ID
Authors

Tatiana Elias Colombo, Ana Carolina Bernardes Terzian, João Pessoa Araújo Júnior, Ricardo Parreira, Eliana Márcia Sotello Cabrera, Izalco Nuremberg Penha dos Santos, Andréia Francesli Negri Reis, Fabiana Rodrigues Costa, Lilian Elisa Arão Antônio Cruz, Patrícia Lopes Rombola, Maurício Lacerda Nogueira

Abstract

Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan(®) RT-qPCR assay. We used a SYBR(®) Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan(®) RT-qPCR assay, 100% (Kappa=0.670) were also found to be negative by SYBR(®) Green RT-qPCR based on Tm comparison; however, 14% (Kappa=0.035) were found to be positive by conventional PCR followed by agarose gel electrophoresis. The differences between the ZIKV strains circulating worldwide and the low viremia period can compromise diagnostic accuracy and thereby the accuracy of outbreak data. Therefore, improved assays are required to improve the diagnosis and surveillance of arbovirus.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 98 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 98 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 19 19%
Student > Master 18 18%
Researcher 8 8%
Student > Ph. D. Student 8 8%
Student > Doctoral Student 6 6%
Other 20 20%
Unknown 19 19%
Readers by discipline Count As %
Immunology and Microbiology 16 16%
Medicine and Dentistry 14 14%
Agricultural and Biological Sciences 12 12%
Biochemistry, Genetics and Molecular Biology 12 12%
Business, Management and Accounting 3 3%
Other 18 18%
Unknown 23 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 September 2017.
All research outputs
#10,978,528
of 12,355,257 outputs
Outputs from Brazilian Journal of Microbiology
#327
of 363 outputs
Outputs of similar age
#230,729
of 271,723 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#23
of 33 outputs
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So far Altmetric has tracked 363 research outputs from this source. They receive a mean Attention Score of 2.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.