Title |
The Proteome of Biologically Active Membrane Vesicles from Piscirickettsia salmonis LF-89 Type Strain Identifies Plasmid-Encoded Putative Toxins
|
---|---|
Published in |
Frontiers in Cellular and Infection Microbiology, September 2017
|
DOI | 10.3389/fcimb.2017.00420 |
Pubmed ID | |
Authors |
Cristian Oliver, Mauricio A. Hernández, Julia I. Tandberg, Karla N. Valenzuela, Leidy X. Lagos, Ronie E. Haro, Patricio Sánchez, Pamela A. Ruiz, Constanza Sanhueza-Oyarzún, Marcos A. Cortés, María T. Villar, Antonio Artigues, Hanne C. Winther-Larsen, Ruben Avendaño-Herrera, Alejandro J. Yáñez |
Abstract |
Piscirickettsia salmonis is the predominant bacterial pathogen affecting the Chilean salmonid industry. This bacterium is the etiological agent of piscirickettsiosis, a significant fish disease. Membrane vesicles (MVs) released by P. salmonis deliver several virulence factors to host cells. To improve on existing knowledge for the pathogenicity-associated functions of P. salmonis MVs, we studied the proteome of purified MVs from the P. salmonis LF-89 type strain using multidimensional protein identification technology. Initially, the cytotoxicity of different MV concentration purified from P. salmonis LF-89 was confirmed in an in vivo adult zebrafish infection model. The cumulative mortality of zebrafish injected with MVs showed a dose-dependent pattern. Analyses identified 452 proteins of different subcellular origins; most of them were associated with the cytoplasmic compartment and were mainly related to key functions for pathogen survival. Interestingly, previously unidentified putative virulence-related proteins were identified in P. salmonis MVs, such as outer membrane porin F and hemolysin. Additionally, five amino acid sequences corresponding to the Bordetella pertussis toxin subunit 1 and two amino acid sequences corresponding to the heat-labile enterotoxin alpha chain of Escherichia coli were located in the P. salmonis MV proteome. Curiously, these putative toxins were located in a plasmid region of P. salmonis LF-89. Based on the identified proteins, we propose that the protein composition of P. salmonis LF-89 MVs could reflect total protein characteristics of this P. salmonis type strain. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Switzerland | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 65 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 10 | 15% |
Student > Bachelor | 10 | 15% |
Student > Doctoral Student | 6 | 9% |
Student > Master | 6 | 9% |
Student > Ph. D. Student | 5 | 8% |
Other | 9 | 14% |
Unknown | 19 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 13 | 20% |
Biochemistry, Genetics and Molecular Biology | 12 | 18% |
Veterinary Science and Veterinary Medicine | 8 | 12% |
Immunology and Microbiology | 5 | 8% |
Medicine and Dentistry | 3 | 5% |
Other | 1 | 2% |
Unknown | 23 | 35% |