Title |
MetaGen: reference-free learning with multiple metagenomic samples
|
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Published in |
Genome Biology, October 2017
|
DOI | 10.1186/s13059-017-1323-y |
Pubmed ID | |
Authors |
Xin Xing, Jun S. Liu, Wenxuan Zhong |
Abstract |
A major goal of metagenomics is to identify and study the entire collection of microbial species in a set of targeted samples. We describe a statistical metagenomic algorithm that simultaneously identifies microbial species and estimates their abundances without using reference genomes. As a trade-off, we require multiple metagenomic samples, usually ≥10 samples, to get highly accurate binning results. Compared to reference-free methods based primarily on k-mer distributions or coverage information, the proposed approach achieves a higher species binning accuracy and is particularly powerful when sequencing coverage is low. We demonstrated the performance of this new method through both simulation and real metagenomic studies. The MetaGen software is available at https://github.com/BioAlgs/MetaGen . |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 9 | 15% |
United Kingdom | 6 | 10% |
Netherlands | 3 | 5% |
Japan | 2 | 3% |
Malaysia | 2 | 3% |
France | 2 | 3% |
Ecuador | 2 | 3% |
India | 2 | 3% |
Sweden | 2 | 3% |
Other | 9 | 15% |
Unknown | 21 | 35% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 30 | 50% |
Members of the public | 28 | 47% |
Science communicators (journalists, bloggers, editors) | 2 | 3% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Ireland | 1 | 1% |
Unknown | 94 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 28 | 29% |
Researcher | 21 | 22% |
Student > Bachelor | 10 | 11% |
Student > Master | 10 | 11% |
Professor | 6 | 6% |
Other | 12 | 13% |
Unknown | 8 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 29 | 31% |
Agricultural and Biological Sciences | 20 | 21% |
Computer Science | 15 | 16% |
Immunology and Microbiology | 5 | 5% |
Mathematics | 4 | 4% |
Other | 10 | 11% |
Unknown | 12 | 13% |