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Physiological characterization of secondary metabolite producing Penicillium cell factories

Overview of attention for article published in Fungal Biology and Biotechnology, October 2017
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2 tweeters

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Title
Physiological characterization of secondary metabolite producing Penicillium cell factories
Published in
Fungal Biology and Biotechnology, October 2017
DOI 10.1186/s40694-017-0036-z
Pubmed ID
Authors

Sietske Grijseels, Jens Christian Nielsen, Jens Nielsen, Thomas Ostenfeld Larsen, Jens Christian Frisvad, Kristian Fog Nielsen, Rasmus John Normand Frandsen, Mhairi Workman

Abstract

Penicillium species are important producers of bioactive secondary metabolites. However, the immense diversity of the fungal kingdom is only scarcely represented in industrial bioprocesses and the upscaling of compound production remains a costly and labor intensive challenge. In order to facilitate the development of novel secondary metabolite producing processes, two routes are typically explored: optimization of the native producer or transferring the enzymatic pathway into a heterologous host. Recent genome sequencing of ten Penicillium species showed the vast amount of secondary metabolite gene clusters present in their genomes, and makes them accessible for rational strain improvement. In this study, we aimed to characterize the potential of these ten Penicillium species as native producing cell factories by testing their growth performance and secondary metabolite production in submerged cultivations. Cultivation of the fungal species in controlled submerged bioreactors showed that the ten wild type Penicillium species had promising, highly reproducible growth characteristics in two different media. Analysis of the secondary metabolite production using liquid chromatography coupled with high resolution mass spectrometry proved that the species produced a broad range of secondary metabolites, at different stages of the fermentations. Metabolite profiling for identification of the known compounds resulted in identification of 34 metabolites; which included several with bioactive properties such as antibacterial, antifungal and anti-cancer activities. Additionally, several novel species-metabolite relationships were found. This study demonstrates that the fermentation characteristics and the highly reproducible performance in bioreactors of ten recently genome sequenced Penicillium species should be considered as very encouraging for the application of native hosts for production via submerged fermentation. The results are particularly promising for the potential development of the ten analysed Penicillium species for production of novel bioactive compounds via submerged fermentations.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 57 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 15 26%
Student > Ph. D. Student 12 21%
Student > Master 7 12%
Researcher 7 12%
Professor 2 4%
Other 5 9%
Unknown 9 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 22 39%
Agricultural and Biological Sciences 9 16%
Chemistry 4 7%
Chemical Engineering 3 5%
Engineering 2 4%
Other 6 11%
Unknown 11 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 October 2017.
All research outputs
#7,135,148
of 12,002,078 outputs
Outputs from Fungal Biology and Biotechnology
#39
of 54 outputs
Outputs of similar age
#144,991
of 284,436 outputs
Outputs of similar age from Fungal Biology and Biotechnology
#4
of 6 outputs
Altmetric has tracked 12,002,078 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 54 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.2. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 284,436 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.