Title |
Independent effects of sham laparotomy and anesthesia on hepatic microRNA expression in rats
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Published in |
BMC Research Notes, October 2014
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DOI | 10.1186/1756-0500-7-702 |
Pubmed ID | |
Authors |
Wiebke Werner, Hannes Sallmon, Annekatrin Leder, Steffen Lippert, Anja Reutzel-Selke, Mehmet Haluk Morgül, Sven Jonas, Christof Dame, Peter Neuhaus, John Iacomini, Stefan G Tullius, Igor M Sauer, Nathanael Raschzok |
Abstract |
Studies on liver regeneration following partial hepatectomy (PH) have identified several microRNAs (miRNAs) that show a regulated expression pattern. These studies involve major surgery to access the liver, which is known to have intrinsic effects on hepatic gene expression and may also affect miRNA screening results. We performed two-third PH or sham laparotomy (SL) in Wistar rats to investigate the effect of both procedures on miRNA expression in liver tissue and corresponding plasma samples by microarray and qRT-PCR analyses. As control groups, non-treated rats and rats undergoing anesthesia only were used. |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Finland | 1 | 11% |
Unknown | 8 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
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Lecturer | 2 | 22% |
Researcher | 2 | 22% |
Student > Doctoral Student | 2 | 22% |
Other | 1 | 11% |
Student > Master | 1 | 11% |
Other | 1 | 11% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 4 | 44% |
Agricultural and Biological Sciences | 2 | 22% |
Biochemistry, Genetics and Molecular Biology | 2 | 22% |
Unknown | 1 | 11% |