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Differential expression of porcine microRNAs in African swine fever virus infected pigs: a proof-of-concept study

Overview of attention for article published in Virology Journal, October 2017
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  • Good Attention Score compared to outputs of the same age (66th percentile)
  • High Attention Score compared to outputs of the same age and source (83rd percentile)

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6 tweeters

Citations

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3 Dimensions

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16 Mendeley
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Title
Differential expression of porcine microRNAs in African swine fever virus infected pigs: a proof-of-concept study
Published in
Virology Journal, October 2017
DOI 10.1186/s12985-017-0864-8
Pubmed ID
Authors

Fernando Núñez-Hernández, Lester Josué Pérez, Marta Muñoz, Gonzalo Vera, Francesc Accensi, Armand Sánchez, Fernando Rodríguez, José I. Núñez

Abstract

African swine fever (ASF) is a re-expanding devastating viral disease currently threatening the pig industry worldwide. MicroRNAs are a class of 17-25 nucleotide non- coding RNAs that have been shown to have critical functions in a wide variety of biological processes, such as cell differentiation, cell cycle regulation, carcinogenesis, apoptosis, regulation of immunity as well as in viral infections by cleavage or translational repression of mRNAs. Nevertheless, there is no information about miRNA expression in an ASFV infection. In this proof-of-concept study, we have analyzed miRNAs expressed in spleen and submandibular lymph node of experimentally infected pigs with a virulent (E75) or its derived attenuated (E75CV1) ASFV strain, as well as, at different times post-infection with the virulent strain, by high throughput sequencing of small RNA libraries. Spleen presented a more differential expression pattern than lymph nodes in an ASFV infection. Of the most abundant miRNAs, 12 were differentially expressed in both tissues at two different times in infected animals with the virulent strain. Of these, miR-451, miR-145-5p, miR-181a and miR-122 presented up-regulation at late times post-infection while miR-92a, miR-23a, miR-92b-3p, miR-126-5p, miR-126-3p, miR-30d, miR-23b and miR-92c showed down-regulation. Of the 8 differentially expressed miRNAs identified at the same time post-infection in infected animals with the virulent strain compared with animals infected with its attenuated strain, miR-126-5p, miR-92c, miR-92a, miR-30e-5p and miR-500a-5p presented up-regulation whereas miR-125b, miR-451 and miR-125a were down-regulated. All these miRNAs have been shown to be associated with cellular genes involved in pathways related to the immune response, virus-host interactions as well as with several viral genes. The study of miRNA expression will contribute to a better understanding of African swine fever virus pathogenesis, essential in the development of any disease control strategy.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 38%
Student > Master 5 31%
Student > Ph. D. Student 2 13%
Unspecified 2 13%
Student > Doctoral Student 1 6%
Other 0 0%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 38%
Veterinary Science and Veterinary Medicine 3 19%
Immunology and Microbiology 3 19%
Earth and Planetary Sciences 1 6%
Unspecified 1 6%
Other 2 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 June 2018.
All research outputs
#3,723,491
of 13,480,725 outputs
Outputs from Virology Journal
#423
of 2,193 outputs
Outputs of similar age
#102,220
of 311,877 outputs
Outputs of similar age from Virology Journal
#35
of 215 outputs
Altmetric has tracked 13,480,725 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 2,193 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 311,877 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 215 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.