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A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni

Overview of attention for article published in Memórias do Instituto Oswaldo Cruz, September 2013
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Title
A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
Published in
Memórias do Instituto Oswaldo Cruz, September 2013
DOI 10.1590/0074-0276108062013006
Pubmed ID
Authors

Marina de Moraes Mourao, Maina Bitar, Francisco Pereira Lobo, Ana Paula Peconick, Priscila Grynberg, Francisco Prosdocimi, Michael Waisberg, Gustavo Coutinho Cerqueira, Andrea Mara Macedo, Carlos Renato Machado, Timothy Yoshino, Gloria Regina Franco

Abstract

Schistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of trans-spliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different libraries. The accessions for each library and for the individual sequences are as follows: (i) adult worms of mixed sexes (LIBEST_027999: JZ139310 - JZ139779), (ii) female adult worms (LIBEST_028000: JZ139780 - JZ140379), (iii) male adult worms (LIBEST_028001: JZ140380 - JZ141002), (iv) eggs (LIBEST_028002: JZ141003 - JZ141497) and (v) schistosomula (LIBEST_028003: JZ141498 - JZ141974).

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 3%
Australia 1 3%
Unknown 30 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 28%
Student > Ph. D. Student 5 16%
Student > Master 5 16%
Professor > Associate Professor 3 9%
Other 2 6%
Other 4 13%
Unknown 4 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 15 47%
Biochemistry, Genetics and Molecular Biology 8 25%
Medicine and Dentistry 2 6%
Environmental Science 1 3%
Unknown 6 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 October 2014.
All research outputs
#11,127,489
of 12,511,869 outputs
Outputs from Memórias do Instituto Oswaldo Cruz
#752
of 862 outputs
Outputs of similar age
#174,783
of 213,720 outputs
Outputs of similar age from Memórias do Instituto Oswaldo Cruz
#13
of 15 outputs
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So far Altmetric has tracked 862 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.