Title |
trieFinder: an efficient program for annotating Digital Gene Expression (DGE) tags
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Published in |
BMC Bioinformatics, October 2014
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DOI | 10.1186/1471-2105-15-329 |
Pubmed ID | |
Authors |
Gabriel Renaud, Matthew C LaFave, Jin Liang, Tyra G Wolfsberg, Shawn M Burgess |
Abstract |
Quantification of a transcriptional profile is a useful way to evaluate the activity of a cell at a given point in time. Although RNA-Seq has revolutionized transcriptional profiling, the costs of RNA-Seq are still significantly higher than microarrays, and often the depth of data delivered from RNA-Seq is in excess of what is needed for simple transcript quantification. Digital Gene Expression (DGE) is a cost-effective, sequence-based approach for simple transcript quantification: by sequencing one read per molecule of RNA, this technique can be used to efficiently count transcripts while obviating the need for transcript-length normalization and reducing the total numbers of reads necessary for accurate quantification. Here, we present trieFinder, a program specifically designed to rapidly map, parse, and annotate DGE tags of various lengths against cDNA and/or genomic sequence databases. |
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Other | 0 | 0% |
Unknown | 1 | 5% |