| Chapter title |
Gel Mobility Shift Assays to Detect Protein-RNA Interactions.
|
|---|---|
| Chapter number | 12 |
| Book title |
Bacterial Regulatory RNA
|
| Published in |
Methods in molecular biology, January 2012
|
| DOI | 10.1007/978-1-61779-949-5_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-948-8, 978-1-61779-949-5
|
| Authors |
Alexander V. Yakhnin, Helen Yakhnin, Paul Babitzke |
| Abstract |
The gel mobility shift assay is a powerful technique for detecting and quantifying protein-RNA interactions. While other techniques such as filter binding and isothermal titration calorimetry (ITC) are available for quantifying protein-RNA interactions, gel shift analysis provides the added advantage that you can visualize the protein-RNA complexes. In the gel shift assay, protein-RNA complexes are typically separated from the unbound RNA using native polyacrylamide gels in Tris/borate/EDTA buffer, although an alternative Tris-glycine buffering system is superior in many situations. Here, we describe both gel shift methods, along with strategies to improve separation of protein-RNA complexes from free RNA, which can be a particular challenge for small RNA binding proteins. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 73 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 16% |
| Student > Bachelor | 11 | 15% |
| Student > Ph. D. Student | 10 | 14% |
| Student > Doctoral Student | 8 | 11% |
| Student > Master | 8 | 11% |
| Other | 7 | 10% |
| Unknown | 17 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 27 | 37% |
| Agricultural and Biological Sciences | 21 | 29% |
| Immunology and Microbiology | 2 | 3% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 3% |
| Chemistry | 2 | 3% |
| Other | 3 | 4% |
| Unknown | 16 | 22% |